Anti-LAMTOR1抗体(ab121157)
Key features and details
- Rabbit polyclonal to LAMTOR1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LAMTOR1抗体
参阅全部 LAMTOR1 一抗 -
描述
兔多克隆抗体to LAMTOR1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to amino acids 9-156 of Human LAMTOR1.
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阳性对照
- WB: HeLa, MCF7 and A549 cell lysates. IHC-P: Human skin, prostate, duodenum and tonsil tissue. A549 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121157于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. -
序列相似性
Belongs to the LAMTOR1 family. -
细胞定位
Late endosome membrane. Lysosome membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 55004 Human
- Entrez Gene: 66508 Mouse
- Entrez Gene: 308869 Rat
- Omim: 613510 Human
- SwissProt: Q6IAA8 Human
- SwissProt: Q9CQ22 Mouse
- SwissProt: Q6P791 Rat
- Unigene: 530753 Human
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别名
- C11orf59 antibody
- LAMTOR1 antibody
- Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1 antibody
see all
图片
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Western blot - Anti-LAMTOR1 antibody (ab121157)All lanes : Anti-LAMTOR1 antibody (ab121157) at 0.04 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMTOR1 knockout HeLa cell lysate
Lane 3 : PANC-1 cell lysate
Lane 4 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 18 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab121157 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab121157 was shown to react with LAMTOR1 in wild-type HeLa cells in Western blot with loss of signal observed in LAMTOR1 knockout cell line ab265351 (LAMTOR1 knockout cell lysate ab258938). Wild-type HeLa and LAMTOR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab121157 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 0.04 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR1 antibody (ab121157)Immunohistochemistry analysis of human tonsil tissue labeling LAMTOR1 with ab121157 at 1/50 dilution. Strong positivity in non-germinal centre cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR1 antibody (ab121157)Immunohistochemistry analysis of human duodenum tissue labeling LAMTOR1 with ab121157 at 1/50 dilution. Strong positivity in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR1 antibody (ab121157)Immunohistochemistry analysis of human skin tissue labeling LAMTOR1 with ab121157 at 1/50 dilution. Strong positivity in squamous epithelial cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR1 antibody (ab121157)Immunohistochemistry analysis of human prostate tissue labeling LAMTOR1 with ab121157 at 1/50 dilution. Strong positivity in glandular cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121157 尚未被引用在任何文献中。