Anti-LAMP2B抗体- Lysosome Marker (ab18529)
Key features and details
- Rabbit polyclonal to LAMP2B - Lysosome Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LAMP2B抗体- Lysosome Marker
参阅全部 LAMP2B 一抗 -
描述
兔多克隆抗体to LAMP2B - Lysosome Marker -
宿主
Rabbit -
特异性
This product has low sequence homology with Mouse LAMP2B (67%), and high sequence homology with Mouse LAMP2C (91.7%). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Cow -
免疫原
Synthetic peptide corresponding to Human LAMP2B aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C which are expressed in different tissues and differ in sequence at the extreme C-terminus.
Database link: P13473
(Peptide available asab23321) -
阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa. It also gave a positive signal in Human skin tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
靶标
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功能
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
组织特异性
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
疾病相关
Danon disease -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3920 Human
- Omim: 309060 Human
- SwissProt: P13473 Human
- Unigene: 496684 Human
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形式
Alternative splicing produces 3 isoforms. -
别名
- CD_antigen=CD107b antibody
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
see all
图片
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All lanes : Anti-LAMP2B antibody - Lysosome Marker (ab18529) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 22 kDa (possible non-specific binding), 33 kDa (possible non-specific binding), 34 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The blot was incubated with ab18529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
LAMP2B undergoes extensive glycosylation so ab18529 detects a band of 110 kDa by Western blot.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab18529 被引用在 28 文献中.
- Zhao S et al. Engineering exosomes derived from subcutaneous fat MSCs specially promote cartilage repair as miR-199a-3p delivery vehicles in Osteoarthritis. J Nanobiotechnology 21:341 (2023). PubMed: 37736726
- Chen F et al. Placental homing peptide guides HIF1α‑silenced exosomes conjugates for targeted enhancement of invasion of trophoblast cells. Mol Med Rep 28:N/A (2023). PubMed: 37232338
- Tran PX et al. Potential for reversing miR-634-mediated cytoprotective processes to improve efficacy of chemotherapy against oral squamous cell carcinoma. Mol Ther Oncolytics 24:897-908 (2022). PubMed: 35571376
- Liu Q et al. iRGD-modified exosomes-delivered BCL6 siRNA inhibit the progression of diffuse large B-cell lymphoma. Front Oncol 12:822805 (2022). PubMed: 35982974
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270