Anti-LAMP2抗体[GL2A7] (ab13524)
![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.cn/ps/products/13/ab13524/Images/ab13524-397579-AntiLAMP2-antibody-GL2A7-western-blot-nih3t3-hek293-human-mouse.jpg "Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)")
Key features and details
- Rat monoclonal [GL2A7] to LAMP2
- Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
概述
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产品名称
Anti-LAMP2抗体[GL2A7]
参阅全部 LAMP2 一抗 -
描述
大鼠单克隆抗体[GL2A7] to LAMP2 -
宿主
Rat -
经测试应用
适用于: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rabbit, Human -
免疫原
Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.
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阳性对照
- WB: HEK-293 and NIH/3T3 cell lysate.
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常规说明
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
GL2A7 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13524于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview. |
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
(PubMed:17425404) |
| IP |
Use at an assay dependent concentration.
(PMID 2142158). |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (7) |
1/500.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
IHC-FoFr
Use at an assay dependent concentration. (PubMed:17425404) |
|
IP
Use at an assay dependent concentration. (PMID 2142158). |
|
Flow Cyt
Use at an assay dependent concentration. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/500. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
组织特异性
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
疾病相关
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3920 Human
- Entrez Gene: 16784 Mouse
- Omim: 309060 Human
- SwissProt: P13473 Human
- SwissProt: P17047 Mouse
- Unigene: 496684 Human
- Unigene: 486 Mouse
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形式
Alternative splicing produces 3 isoforms. -
别名
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
图片
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Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
Secondary
All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilutionBlock: 5% milk + TBST for 1 hour at RT.
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![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.cn/ps/products/13/ab13524/Images/ab13524-260757-anti-lamp2-antibody-gl2a7-western-blot.jpg)
Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
Secondary
Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 3 minutesBlocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (207)
ab13524 被引用在 207 文献中.
- Zhang X et al. Use of acidic nanoparticles to rescue macrophage lysosomal dysfunction in atherosclerosis. Autophagy 19:886-903 (2023). PubMed: 35982578
- Park K et al. Impaired TFEB activation and mitophagy as a cause of PPP3/calcineurin inhibitor-induced pancreatic β-cell dysfunction. Autophagy 19:1444-1458 (2023). PubMed: 36217215
- Walter DM et al. Setd2 inactivation sensitizes lung adenocarcinoma to inhibitors of oxidative respiration and mTORC1 signaling. Commun Biol 6:255 (2023). PubMed: 36899051
- Kumar S et al. pH regulates hematopoietic stem cell potential via polyamines. EMBO Rep 24:e55373 (2023). PubMed: 36943011
- Zhang J et al. Analgesic and potentiated photothermal therapy with ropivacaine-loaded hydrogels. Theranostics 13:2226-2240 (2023). PubMed: 37153743