重组Anti-LAMP1抗体[EPR4204] - Lysosome Marker (ab108597)
![Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-513331-anti-mamp1-antibody-epr4204-western-blot-wildtype-hct-116-lamp1-knockout-hct-116-hela-mcf7.jpg "Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4204] to LAMP1 - Lysosome Marker
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LAMP1抗体[EPR4204] - Lysosome Marker
参阅全部 LAMP1 一抗 -
描述
兔单克隆抗体[EPR4204] to LAMP1 - Lysosome Marker -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, JAR, A431 and Jurkat lysates. IHC-P: human liver carcinoma and kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4204 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
- PE Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318365)
- APC Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318468)
- Alexa Fluor® 488 Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318571)
- Alexa Fluor® 647 Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318674)
- Alexa Fluor® 594 Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318777)
- Alexa Fluor® 555 Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab318877)
- Alexa Fluor® 750 Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab320909)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108597于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
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| IHC-P | (1) |
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa). |
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IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3916 Human
- Omim: 153330 Human
- SwissProt: P11279 Human
- Unigene: 494419 Human
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别名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
图片
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Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 cell lysate
Lane 2 : LAMP1 knockout HCT 116 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 100-120 kDa why is the actual band size different from the predicted?Western blot: Anti-LAMP1 antibody [EPR4204] - Lysosome Marker staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108597 was shown to bind specifically to LAMP1. A band was observed at 100-120 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in LAMP1 knockout cell line. To generate this image, wild-type and LAMP1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-477522-a.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20µg
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 20µg
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 20µg
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsLAMP1 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-4-anti-lamp1-antibody-epr4204-immunohistochemistry-liver-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-304630-anti-lamp1-antibody-epr4204-western-blot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LAMP1 knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : Human Kidney whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-121295-anti-lamp1-antibody-epr4204-western-blot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution ((unpurified))
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-121294-anti-lamp1-antibody-epr4204-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)](https://www.abcam.cn/ps/products/108/ab108597/Images/ab108597-6-benefits-of-recombinant-antibodies.png)
Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab108597 被引用在 14 文献中.
- Tai S et al. Metformin suppresses vascular smooth muscle cell senescence by promoting autophagic flux. J Adv Res 41:205-218 (2022). PubMed: 36328749
- Zhao X et al. Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice. Front Cell Neurosci 16:1021592 (2022). PubMed: 36439204
- Yang Z et al. miR-155-5p in Extracellular Vesicles Derived from Choroid Plexus Epithelial Cells Promotes Autophagy and Inflammation to Aggravate Ischemic Brain Injury in Mice. Oxid Med Cell Longev 2022:8603427 (2022). PubMed: 35222806
- Jiao J et al. LACC1 contributes to inflammation and cognitive disorder after stroke via the AMPK/NLRP3 pathway. Acta Neurobiol Exp (Wars) 82:207-212 (2022). PubMed: 35833820
- Wang W et al. Circ-SIRT1 inhibits cardiac hypertrophy via activating SIRT1 to promote autophagy. Cell Death Dis 12:1069 (2021). PubMed: 34759275