AB108536

重组Anti-Laminin beta 1抗体[EPR3189(2)]

Anti-Laminin beta 1 antibody [EPR3189(2)]

RabMAb
Recombinant
KO Validated
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(8 Publications)

Rabbit Recombinant Monoclonal LAMB1 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 8 publications.

查看别名

Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

2 Images

Lab

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (AB108536)

Lanes 1- 2 : Merged signal (red and green). Green - ab108536 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab108536 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108536 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 1:

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (<a href='/products/cell-lysates/human-lamb1-laminin-beta-1-knockout-hela-cell-lysate-ab257499'>ab257499</a>)

Lane 2:

LAMB1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell line (<a href='/products/cell-lines/human-lamb1-laminin-beta-1-knockout-hela-cell-line-ab265414'>ab265414</a>)

Predicted band size: 198 kDa

Observed band size: 200 kDa

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Unknown

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (AB108536)

All lanes:

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution

Lane 1:

JAR cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Predicted band size: 198 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3189(2)

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 1 also known as LAMB1 is a subunit of the laminin molecule an essential component of the extracellular matrix. This protein has an approximate molecular weight of 200 kDa. Laminin beta 1 is one of the three chains that form the laminin heterotrimer. It is predominantly expressed in basement membranes which provide structural support and segregate tissues. The laminin 1 (laminin-111) complex including the LAMB1 chain contributes to cell adhesion differentiation migration and plays a role in tissue development and repair.

Biological function summary

This component of the laminin molecule plays a critical role in maintaining the integrity of tissues by organizing cell architecture and mediating cellular signals. Laminin beta 1 is part of a larger laminin protein complex that interacts with a variety of cell surface receptors including integrins and dystroglycan contributing to cell communication and signal transduction. These interactions guide cell orientation and regulate vital processes such as cell movement and growth.

Pathways

Laminin beta 1 participates in the integrin signaling pathway and the PI3K-AKT signaling pathway. It interacts closely with integrin proteins such as integrin alpha-6 and integrin beta-4 during these processes. In integrin signaling the laminin molecule facilitates the binding between cells and their surrounding extracellular matrix. This connection influences cellular responses including survival and motility which are necessary for normal development and wound healing processes.

Alterations in laminin beta 1 expression are linked to conditions like muscular dystrophy and Pierson syndrome. In muscular dystrophy the dysfunction of laminin beta 1 interacts with proteins such as dystrophin in muscle cells contributing to compromised structural integrity and muscle wasting. Similarly in Pierson syndrome abnormalities in laminin beta 1 are associated with a rare genetic disorder that affects the kidneys and eyes where it implicates the protein nephrin in the glomerular basement membrane leading to serious renal dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (PubMed : 23472759). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).

See full target information LAMB1

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29297 PubMed40789880

2025

Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Yuan Gao,Kadirya Asan,Jing Ji,Jinjin Xu,Xiaoyan Wu,Jie Ren,Wen Feng,Mengwei Song,Sen Wang,Boyu Zhang,Ying Zhou,Conghui Han,Shun Liu,Wen Yang

Frontiers in bioengineering and biotechnology 12:1481295 PubMed39664883

2024

Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Houlong Long,Peng Zhang,Bin Liu,Shuheng Li,Rong Sun,Tongmei Diao,Feng Li

International journal of molecular sciences 25: PubMed39518978

2024

miR-30c-5p Gain and Loss of Function Modulate Sciatic Nerve Injury-Induced Nucleolar Stress Response in Dorsal Root Ganglia Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Francés,Jorge Mata-Garrido,Miguel Lafarga,María A Hurlé,Mónica Tramullas

Materials today. Bio 28:101235 PubMed39318374

2024

Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomu Ma,Qiang Yue,Su Fu,Chunjun Liu,Jie Luan

Frontiers in immunology 12:666361 PubMed34168643

2021

M2a Macrophage-Secreted CHI3L1 Promotes Extracellular Matrix Metabolic Imbalances Activation of IL-13Rα2/MAPK Pathway in Rat Intervertebral Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Long Li,Kang Wei,Yifan Ding,Paerxiati Ahati,Haoran Xu,Huang Fang,Huan Wang

Bioengineering (Basel, Switzerland) 6: PubMed31817298

2019

Corneal Epithelial-Stromal Fibroblast Constructs to Study Cell-Cell Communication in Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tina B McKay,Dimitrios Karamichos,Audrey E K Hutcheon,Xiaoqing Guo,James D Zieske

International journal of molecular sciences 20: PubMed31426484

2019

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenza Bacci,Aurora Aiello,Cristian Ripoli,Rossella Loria,Dario Pugliese,Francesco Pierconti,Dante Rotili,Lidia Strigari,Francesco Pinto,Pier Francesco Bassi,Antonello Mai,Claudio Grassi,Alfredo Pontecorvi,Rita Falcioni,Antonella Farsetti,Simona Nanni

Proteomics 19:e1800334 PubMed30908848

2019

Multi-Omics Profiling for NF1 Target Discovery in Neurofibromin (NF1) Deficient Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel M Carnes,James A Mobley,David K Crossman,Hui Liu,Bruce R Korf,Robert A Kesterson,Deeann Wallis

View all publications

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重组Anti-Laminin beta 1抗体[EPR3189(2)]