重组Anti-Laminin beta 1抗体[EPR23265-32] - BSA and Azide free (ab273053)
![Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.cn/ps/products/273/ab273053/Images/ab273053-2-anti-laminin-beta-1-antibody-epr2326532-immunocytochemistry-hepg2.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23265-32] to Laminin beta 1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Laminin beta 1抗体[EPR23265-32] - BSA and Azide free
参阅全部 Laminin beta 1 一抗 -
描述
兔单克隆抗体[EPR23265-32] to Laminin beta 1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: Flow Cyt,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: NIH/3T3 and HepG2 cells.
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常规说明
ab273053 is the carrier-free version of ab256380.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23265-32 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 198 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 198 kDa. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
序列相似性
Contains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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数据库链接
- Entrez Gene: 3912 Human
- Entrez Gene: 16777 Mouse
- Entrez Gene: 298941 Rat
- Omim: 150240 Human
- SwissProt: P07942 Human
- SwissProt: P02469 Mouse
- Unigene: 650585 Human
- Unigene: 172674 Mouse
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别名
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Laminin beta 1 with ab256380 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256380).
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![Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.cn/ps/products/273/ab273053/Images/ab273053-1-anti-laminin-beta-1-antibody-epr2326532-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Laminin beta 1 with ab256380 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256380).
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![Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.cn/ps/products/273/ab273053/Images/ab273053-3-benefits-of-recombinant-antibodies.png)
Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273053 尚未被引用在任何文献中。
