Anti-Laminin alpha 5/LAMA5抗体[4C7] (ab17107)

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however tested in WB so the guarantee will apply to WB only.

I also have read the details you have kindly provided and have following further questions for better understanding of the problem;

- What is the purchase order number? The number NL/Abcam 2698(N) does not match to any info we have in system.
- Are all the three product failed?
- What were the storage conditions of products?
- Did you observe non specific staining, no staining or low signal?
- Could you provide the species and type of tissues sections? e.g. human lungs
- How the sections were fixed?
- What sort of antigen retrieval was used? e.g. microwave with citrate buffer pH 6.0.
- Have you tried any permeabilization step?
- Did you try blocking the tissue sections?
- What was the species and conjugate of secondary antibodies? Have you used these successfully before?
- Did you try any sort of troubleshooting?
- Could you provide the images?

In order to get the result soon please provide all the requested details .Any additional information will also be helpful.

I will look forward to hearing from you soon.

Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain is the C-terminal portion of the A chain and the 4C7 antibody could therefore be against the A chain." Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 If you have any additional questions, please contact us again.

This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 Kleinman HK et al. Laminin receptors for neurite formation. Proc Natl Acad Sci U S A 85:1282-6 (1988). PubMed: 2963341 If you have any additional questions, please contact us again.