Anti-Laminin alpha 5/LAMA5抗体[4B12] (ab77175)
![Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (ab77175)](https://www.abcam.cn/ps/products/77/ab77175/Images/ab77175-172796-ab77175.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (ab77175)")
Key features and details
- Mouse monoclonal [4B12] to Laminin alpha 5/LAMA5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Laminin alpha 5/LAMA5抗体[4B12]
参阅全部 Laminin alpha 5/LAMA5 一抗 -
描述
小鼠单克隆抗体[4B12] to Laminin alpha 5/LAMA5 -
宿主
Mouse -
经测试应用
适用于: ICC/IFmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.
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阳性对照
- Human embryonic lung (alveolar epithelium). Human kidney.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
4B12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77175于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
组织特异性
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
序列相似性
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
结构域
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
细胞定位
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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数据库链接
- Entrez Gene: 3911 Human
- Omim: 601033 Human
- SwissProt: O15230 Human
- Unigene: 473256 Human
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别名
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (ab77175)
Immunofluorescence of (A) Human embryonic lung alveolae epithelium and (B) Human kidney labelling Lamin alpha 5 with ab77175.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab77175 被引用在 10 文献中.
- Phan V et al. Molecular mechanisms in chloroquine-exposed muscle cells elucidated by combined proteomic and microscopic studies. Neuropathol Appl Neurobiol 49:e12877 (2023). PubMed: 36633103
- Li L et al. Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling. Commun Biol 5:155 (2022). PubMed: 35197546
- Le-Bel G et al. Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells. Cells 11:N/A (2022). PubMed: 36078126
- Majmundar AJ et al. Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Sci Adv 7:N/A (2021). PubMed: 33523862
- Tuffin J et al. GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening. Commun Biol 4:1351 (2021). PubMed: 34857869
- Ma C et al. Differential Labeling of Glycoproteins with Alkynyl Fucose Analogs. Int J Mol Sci 21:N/A (2020). PubMed: 32825463
- Tsai S et al. Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models. BMC Cancer 18:335 (2018). ICC/IF . PubMed: 29587663
- Hale LJ et al. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening. Nat Commun 9:5167 (2018). PubMed: 30514835
- Huang L et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med 21:1364-71 (2015). PubMed: 26501191
- Shimshek DR et al. The HSP70 molecular chaperone is not beneficial in a mouse model of alpha-synucleinopathy. PLoS One 5:e10014 (2010). PubMed: 20368804