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AB210957

Anti-Laminin alpha 5/LAMA5 抗体 [CL3118]

Anti-Laminin alpha 5/LAMA5 antibody [CL3118]

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(2 Publications)

Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAMA5 aa 2550 to C-terminus.

查看别名

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue (negative control), labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human cervix tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human heart tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • WB

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Laminin alpha 5/LAMA5 antibody. Remaining relative intensity is presented. Loading control : Anti-GAPDH (36 kDa).

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1 µg/mL

Lane 1:

Ctrl siRNA

Lane 2:

LAMA5 siRNA #1

Lane 3:

LAMA5 siRNA #2

Secondary

All lanes:

HRP-conjugated Goat Anti-Mouse Immunoglobulin at 1/3000 dilution

Predicted band size: 400 kDa

Observed band size: 36 kDa

false

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)
  • WB

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution

Lane 1:

Recombinant protein fragment of Human Laminin 511

Lane 2:

Recombinant protein fragment of Human Laminin 521

Lane 3:

Recombinant protein fragment of Human Laminin 121

Lane 4:

Recombinant protein fragment of Human Laminin 221

Lane 5:

Recombinant protein fragment of Human Laminin 332

Lane 6:

Recombinant protein fragment of Human Laminin 411

Predicted band size: 400 kDa

true

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CL3118

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human LAMA5 aa 2550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15230

表位

ab210957 binds to an epitope located within the peptide sequence RQGLVDRAQQ (aa 2555 - 2564).

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替代产品

Primary Antibodies

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Anti-Laminin alpha 5/LAMA5 antibody [4B12]

Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (AB77175)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody (AB238582)

  • 0
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Primary Antibodies

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Anti-Laminin alpha 5/LAMA5 antibody

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AB184330

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]

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Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

  • 4
  • 2
Primary Antibodies

AB271947

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (AB271947)

  • 0
  • 0
Primary Antibodies

AB17107

Anti-Laminin alpha 5/LAMA5 antibody [4C7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

  • 4
  • 3

搭配使用产品

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

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AB179471

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] (AB179471)

  • 5
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Primary Antibodies

AB6586

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

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Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p>Because Laminin alpha 5/LAMA5 expression levels can vary significantly across different tissue types, negative staining in IHC-P may result from low antigen expression. In cases where no signal is detected, using an HRP polymer-conjugated secondary antibody and DAB-based detection is recommended to enhance signal amplification and assay sensitivity.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.
Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).
See full target information LAMA5
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:7902-7914 PubMed38709270

2024

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dayong Du,Yan Huang,Cui Xu,Yang Liu,Xiaohong Wu,Jie Yang,Zhipeng Liu,Jianxin Ma,Chaoji Huangfu

Clinical kidney journal 17:sfae037 PubMed38455522

2024

Characterization of glomerular basement membrane components within pediatric glomerular diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Xindi Zhou,Chun Gan,Qing Yang,Wanbing Chen,Xiaoqian Feng,Tao Zhang,Li Zhang,Lujun Dai,Yaxi Chen,Haiping Yang,Mo Wang,Wei Jiang,Qiu Li
View all publications
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