AB17107

Anti-Laminin alpha 5/LAMA5 抗体 [4C7]

Name: Laminin alpha 5/LAMA5抗体[4C7] (ab17107)| Abcam中文官网
Brand: Abcam Limited
SKU: AB17107
Price: 2969 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Laminin alpha 5/LAMA5 antibody [4C7]

(3 Reviews)

(3 Publications)

Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LAMA5.

查看别名

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

IHC-P

AbReview7555****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

This image was generated using the ascites version of the product.

This image is courtesy of an anonymous Abreview

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5/LAMA5 with ab17107.

This image was generated using the ascites version of the product.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4C7

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

Native Full Length Protein corresponding to Human LAMA5.

O15230

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5 - 1/20", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5 - 1/20", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5 - 1/20", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

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产品详情

This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.

Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

See full target information LAMA5

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40304687

2025

Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuki Isogai,Koichiro M Hirosawa,Miki Kanno,Ayano Sho,Rinshi S Kasai,Naoko Komura,Hiromune Ando,Keiko Furukawa,Yuhsuke Ohmi,Koichi Furukawa,Yasunari Yokota,Kenichi G N Suzuki

Cancer science 111:3478-3492 PubMed32648337

2020

Novel monoclonal antibody against integrin α3 shows therapeutic potential for ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Yi Ke,Wan-Yu Chen,Ming-Chieh Lin,Yu-Chyi Hwang,Kuan-Ting Kuo,Han-Chung Wu

FASEB journal : official publication of the Federa 25:1943-59 PubMed21368102

2011

Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle.

Applications

IHC-Fr

Species

Human

Abigail L Mackey,Simon Brandstetter,Peter Schjerling,Jens Bojsen-Moller,Klaus Qvortrup,Mette M Pedersen,Simon Doessing,Michael Kjaer,S Peter Magnusson,Henning Langberg

View all publications

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Anti-Laminin alpha 5/LAMA5 抗体 [4C7]

Anti-Laminin alpha 5/LAMA5 antibody [4C7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (AB17107)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原