Anti-Laminin 5抗体(ab14509)
Key features and details
- Rabbit polyclonal to Laminin 5
- Suitable for: WB, ELISA, IHC-Fr, ICC/IF, IP
- Reacts with: Mouse, Horse, Dog, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Laminin 5抗体
参阅全部 Laminin 5 一抗 -
描述
兔多克隆抗体to Laminin 5 -
宿主
Rabbit -
经测试应用
适用于: WB, ELISA, IHC-Fr, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Horse, Dog, Human -
免疫原
This product was produced with the following immunogens:
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q16787
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q13751
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q13753 -
阳性对照
- WB: Cell culture supernatant of keratinocytes. HaCaT cell supernatant. SCC-25 cell supernatant. Purified Laminin 5.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.2% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa.
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ELISA | (1) |
Use at an assay dependent concentration.
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IHC-Fr | (4) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 16940506
|
IP |
Use at an assay dependent concentration. PubMed: 16940506
|
说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 16940506 |
IP
Use at an assay dependent concentration. PubMed: 16940506 |
靶标
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相关性
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. -
数据库链接
- Entrez Gene: 3909 Human
- Entrez Gene: 3914 Human
- Entrez Gene: 3918 Human
- SwissProt: Q13751 Human
- SwissProt: Q13753 Human
- SwissProt: Q16787 Human
- SwissProt: Q61087 Mouse
- SwissProt: Q61092 Mouse
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (71)
ab14509 被引用在 71 文献中.
- Chen J et al. Critical Residues Involved in the Coassembly of L1 and L2 Capsid Proteins of Human Papillomavirus 16. J Virol 97:e0181922 (2023). PubMed: 36815785
- Siljamäki E et al. Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394. Oncogene 42:3633-3647 (2023). PubMed: 37864034
- Skulska K et al. Age-Related Changes in Female Murine Reproductive Mucosa with respect to γδ T Cell Presence. J Immunol Res 2023:3072573 (2023). PubMed: 36726490
- Parigoris E et al. Extended longevity geometrically-inverted proximal tubule organoids. Biomaterials 290:121828 (2022). PubMed: 36215909
- Mezawa M et al. TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells. Cell Adh Migr 16:13-24 (2022). PubMed: 35137648