Anti-Laminin 2 alpha抗体[4H8-2] (ab11576)
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)](https://www.abcam.cn/ps/products/11/ab11576/Images/ab11576-396121-anti-laminin2-alpha-antibody-4h8-2-immunohistochemistry-tongue-mouse.jpg "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)")
Key features and details
- Rat monoclonal [4H8-2] to Laminin 2 alpha
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-Laminin 2 alpha抗体[4H8-2]
参阅全部 Laminin 2 alpha 一抗 -
描述
大鼠单克隆抗体[4H8-2] to Laminin 2 alpha -
宿主
Rat -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Mouse
预测可用于: Human
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免疫原
Full length protein corresponding to Mouse Laminin 2 alpha. Heart Laminin 2 (Mouse).
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表位
The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin. -
阳性对照
- IHC-Fr: Mouse tongue tissue.
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常规说明
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
4H8-2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (5) |
Use a concentration of 4 - 8 µg/ml.
This concentration was determined for acetone-fixed frozen sections of human tongue. |
| 说明 |
|---|
|
IHC-Fr
Use a concentration of 4 - 8 µg/ml. This concentration was determined for acetone-fixed frozen sections of human tongue. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
组织特异性
Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. -
疾病相关
Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI. -
序列相似性
Contains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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数据库链接
- Entrez Gene: 3908 Human
- Entrez Gene: 16773 Mouse
- Omim: 156225 Human
- SwissProt: P24043 Human
- SwissProt: Q60675 Mouse
- Unigene: 200841 Human
- Unigene: 256087 Mouse
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别名
- LAMA 2 antibody
- LAMA2 antibody
- LAMA2_HUMAN antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).
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![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)](https://www.abcam.cn/ps/products/11/ab11576/Images/ab11576-459696-anti-laminin-2-alpha-antibody-4h8-2-immunohistochemistry-frozen-muscle-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an anonymous Abreview.Immunohistochemistry (Frozen sections) analysis of Mouse muscle tissue labeling Laminin 2 alpha with ab11576 at 1/200 dilution. Tissue fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS for 30 minutes. Tissue blocked with 10% goat serum for 1 hour at room temperature followed by incubation with the primary antibody in 2% goat serum, 0.2% triton X-100 in PBS overnight at 4°C. Goat anti-rat IgG (Alexa Fluor® 568) was used as the secondary antibody at 1/500 dilution (red).
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![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)](https://www.abcam.cn/ps/products/11/ab11576/Images/ab11576-19048-anti-laminin-2-alpha-antibody-4h8-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an Abreview submitted by Dr Ling Guoab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (72)
ab11576 被引用在 72 文献中.
- Karreman MA et al. Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization. Cancer Res 83:1299-1314 (2023). PubMed: 36652557
- Increased Dystrophin Production With Golodirsen in Patients With Duchenne Muscular Dystrophy. Neurology 100:936 (2023). PubMed: 37156629
- Hiyoshi T et al. Electrical impedance myography detects dystrophin-related muscle changes in mdx mice. Skelet Muscle 13:19 (2023). PubMed: 37980539
- Desjardins CA et al. Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice. Nucleic Acids Res 50:11401-11414 (2022). PubMed: 35944903
- Biswas S et al. Mural Wnt/β-catenin signaling regulates Lama2 expression to promote neurovascular unit maturation. Development 149:N/A (2022). PubMed: 36098369