Anti-Laminin 2 alpha 抗体 [4H8-2]

• IHC-Fr

AbReview70547****

Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)

Immunohistochemistry (Frozen sections) analysis of Mouse muscle tissue labeling Laminin 2 alpha with ab11576 at 1/200 dilution. Tissue fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS for 30 minutes. Tissue blocked with 10% goat serum for 1 hour at room temperature followed by incubation with the primary antibody in 2% goat serum, 0.2% triton X-100 in PBS overnight at 4°C. Goat anti-rat IgG (Alexa Fluor^® 568) was used as the secondary antibody at 1/500 dilution (red).

This image is courtesy of an anonymous Abreview.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)

Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).

• IHC-Fr

AbReview9848****

Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)

ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Ling Guo