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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix

Anti-Laminin 1+2抗体(ab7463)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (61)

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Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
  • Western blot - Anti-Laminin 1+2 antibody (ab7463)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)
  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

Key features and details

  • Rabbit polyclonal to Laminin 1+2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Laminin 1+2抗体
  • 描述

    兔多克隆抗体to Laminin 1+2
  • 宿主

    Rabbit
  • 特异性

    This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1).
  • 经测试应用

    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) (Mouse EHS tumour).

  • 常规说明


    Laminin antibodies are widely used to label blood vessels and basement membranes.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Laminin antibodies are widely used to label blood vessels and basement membranes.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7463于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C,  especially paraffin tissue.

WB (1)
1/100 - 1/5000.

Use 5-7% of gel for electrophoresis.

ICC/IF (1)
1/50 - 1/200.
说明
IHC-P
1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C,  especially paraffin tissue.

WB
1/100 - 1/5000.

Use 5-7% of gel for electrophoresis.

ICC/IF
1/50 - 1/200.

靶标

  • 相关性

    Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 细胞定位

    Secreted, Basement membrane, Extracellular matrix
  • 数据库链接

    • Entrez Gene: 284217 Human
    • Entrez Gene: 3908 Human
    • Entrez Gene: 3912 Human
    • Entrez Gene: 3913 Human
    • Entrez Gene: 3915 Human
    • Entrez Gene: 3918 Human
    • Entrez Gene: 84823 Human
    • Entrez Gene: 16772 Mouse
    • Entrez Gene: 16773 Mouse
    • Entrez Gene: 16779 Mouse
    • Entrez Gene: 16782 Mouse
    • Entrez Gene: 226519 Mouse
    • Omim: 150240 Human
    • Omim: 150290 Human
    • Omim: 150292 Human
    • Omim: 150320 Human
    • Omim: 150325 Human
    • Omim: 156225 Human
    • SwissProt: P07942 Human
    • SwissProt: P11047 Human
    • SwissProt: P24043 Human
    • SwissProt: P25391 Human
    • SwissProt: Q03252 Human
    • SwissProt: Q13753 Human
    • SwissProt: P02468 Mouse
    • SwissProt: P19137 Mouse
    • SwissProt: Q8R0Y0 Mouse
    see all
  • 别名

    • LAMA antibody
    • LAMA1 antibody
    • LAMA2 antibody
    • LAMB antibody
    • LAMB1 antibody
    • LAMC antibody
    • LAMC1 antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 2 antibody
    • Laminin alpha 2 subunit antibody
    • Laminin B1 chain antibody
    • Laminin B2 chain antibody
    • Laminin beta 1 antibody
    • Laminin gamma 1 antibody
    • Laminin M antibody
    • LAMM antibody
    • Merosin antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
    Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

    Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
    Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

    Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments. 

  • Western blot - Anti-Laminin 1+2 antibody (ab7463)
    Western blot - Anti-Laminin 1+2 antibody (ab7463)
    All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml

    Lane 1 : Mouse EHS tumor crude extracts
    Lane 2 : Rat Heart Crude Extracts

    Observed band size: 200,400 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)
    Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.
  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
    Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

    ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.

实验方案

  • Recommended IHC-P protocol with ab7463
  • Recommended WB protocol with ab7463

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (61)

发表研究结果有使用 ab7463?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7463 被引用在 61 文献中.

  • Rütsche D  et al. Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature. Adv Mater 35:e2209476 (2023). PubMed: 36724374
  • Lu JH  et al. Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat. Int J Mol Sci 24:N/A (2023). PubMed: 37175945
  • Wang Y  et al. Sodium salicylate ameliorates exercise-induced muscle damage in mice by inhibiting NF-kB signaling. J Orthop Surg Res 18:967 (2023). PubMed: 38098039
  • Gómez Atria D  et al. Stromal Notch ligands foster lymphopenia-driven functional plasticity and homeostatic proliferation of naive B cells. J Clin Invest 132:N/A (2022). PubMed: 35579963
  • Sun Y  et al. Ninj2 regulates Schwann cells development by interfering laminin-integrin signaling. Theranostics 12:7307-7318 (2022). PubMed: 36438492
View all Publications for this product

客户评价及客户问答

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Western blot abreview for Anti-Laminin 1+2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (intestinal mucosa)
Loading amount
70 µg
Specification
intestinal mucosa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sofia Tedelind

Verified customer

提交于 Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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