Anti-Laminin 1+2抗体(ab7463)
Key features and details
- Rabbit polyclonal to Laminin 1+2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Laminin 1+2抗体 -
描述
兔多克隆抗体to Laminin 1+2 -
宿主
Rabbit -
特异性
This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1). -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length native protein (purified) (Mouse EHS tumour).
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常规说明
Laminin antibodies are widely used to label blood vessels and basement membranes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Laminin antibodies are widely used to label blood vessels and basement membranes. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C, especially paraffin tissue. |
| WB | (1) |
1/100 - 1/5000.
Use 5-7% of gel for electrophoresis. |
| ICC/IF | (1) |
1/50 - 1/200.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C, especially paraffin tissue. |
|
WB
1/100 - 1/5000. Use 5-7% of gel for electrophoresis. |
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ICC/IF
1/50 - 1/200. |
靶标
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相关性
Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
细胞定位
Secreted, Basement membrane, Extracellular matrix -
数据库链接
- Entrez Gene: 284217 Human
- Entrez Gene: 3908 Human
- Entrez Gene: 3912 Human
- Entrez Gene: 3913 Human
- Entrez Gene: 3915 Human
- Entrez Gene: 3918 Human
- Entrez Gene: 84823 Human
- Entrez Gene: 16772 Mouse
see all -
别名
- LAMA antibody
- LAMA1 antibody
- LAMA2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain.
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments.
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Western blot - Anti-Laminin 1+2 antibody (ab7463)All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml
Lane 1 : Mouse EHS tumor crude extracts
Lane 2 : Rat Heart Crude Extracts
Observed band size: 200,400 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample. -
Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (53)
ab7463 被引用在 53 文献中.
- Gómez Atria D et al. Stromal Notch ligands foster lymphopenia-driven functional plasticity and homeostatic proliferation of naive B cells. J Clin Invest 132:N/A (2022). PubMed: 35579963
- Sun Y et al. Ninj2 regulates Schwann cells development by interfering laminin-integrin signaling. Theranostics 12:7307-7318 (2022). PubMed: 36438492
- Adua SJ et al. Brain metastatic outgrowth and osimertinib resistance are potentiated by RhoA in EGFR-mutant lung cancer. Nat Commun 13:7690 (2022). PubMed: 36509758
- Larouche JA et al. Murine muscle stem cell response to perturbations of the neuromuscular junction are attenuated with aging. Elife 10:N/A (2021). PubMed: 34323217
- Altera A et al. The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina. Graefes Arch Clin Exp Ophthalmol 259:2559-2571 (2021). PubMed: 33760980