Anti-Lamin B2抗体[LN43] (ab8983)
![Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-15967-anti-lamin-b2-antibody-ln43-western-blot.jpg "Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)")
Key features and details
- Mouse monoclonal [LN43] to Lamin B2
- Suitable for: Flow Cyt, IHC-Fr, WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Lamin B2抗体[LN43]
参阅全部 Lamin B2 一抗 -
描述
小鼠单克隆抗体[LN43] to Lamin B2 -
宿主
Mouse -
特异性
This antibody reacts with an epitope located in the C-terminal part of lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. Please see the listed positive controls for this antibody. Other cell/tissue types have not been tested. -
经测试应用
适用于: Flow Cyt, IHC-Fr, WB, IPmore details -
种属反应性
与反应: Human, Zebrafish -
免疫原
Tissue, cells or virus corresponding to Lamin B2. Detergent insoluble fraction of potoroo cell line PtK1.
Database link: Q03252 -
表位
Epitope located in the C-terminal part of lamin B2. -
阳性对照
- IHC-Fr: testicular germ cell tumour sections (see Machiels et al 1997), parabasal cells of skin epidermis (see Broers et al). WB: NCI-H125 nuclear cell lysate Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
LN43 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr |
1/1.
(when used with an avidin-biotinylated HRP complex as detection agent). |
|
| WB | (2) |
1/100 - 1/1000. Detects a band of approximately 65-67 kDa.
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
1/1. (when used with an avidin-biotinylated HRP complex as detection agent). |
|
WB
1/100 - 1/1000. Detects a band of approximately 65-67 kDa. |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
疾病相关
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
细胞定位
Nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 84823 Human
- Omim: 150341 Human
- SwissProt: Q03252 Human
- Unigene: 728836 Human
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别名
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
图片
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Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)All lanes : Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-391629-anti-lamin-b2-antibody-ln43-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)ab8983 immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-218486-anti-lamin-b2-antibody-ln43-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.
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![Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-15965-anti-lamin-b2-antibody-ln43-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 73kDa: Lamin B2 -
![Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-15966-anti-lamin-b2-antibody-ln43-flow-cytometry.jpg)
Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.cn/ps/products/8/ab8983/Images/ab8983-218487-anti-lamin-b2-antibody-ln43-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)IF staining of a 9 days old zebrafish embryo.
实验方案
数据表及文件
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Datasheet download
文献 (46)
ab8983 被引用在 46 文献中.
- Balcazar Lopez CE et al. Alternative promoters and splicing create multiple functionally distinct isoforms of oestrogen receptor alpha in breast cancer and healthy tissues. Cancer Med 12:18931-18945 (2023). PubMed: 37676103
- van Schaik T et al. Genome-Wide Mapping and Microscopy Visualization of Protein-DNA Interactions by pA-DamID. Methods Mol Biol 2458:215-229 (2022). PubMed: 35103970
- Fathi A et al. Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegeneration. Aging Cell 21:e13541 (2022). PubMed: 34953016
- LaJoie D et al. A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents. Mol Biol Cell 33:ar117 (2022). PubMed: 36044344
- Hauser N et al. Exploiting the Endogenous Ubiquitin Proteasome System in Targeted Cancer Treatment. Cancers (Basel) 15:N/A (2022). PubMed: 36612252
