Anti-Lamin B1 抗体 [119D5-F1] - Nuclear Envelope Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

ab8982 staining Lamin B1 in wild-type HAP1 cells (top panel) and LMNB1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8982 at 0.5μg/ml and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Characteriser

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Methanol fixed HeLa and 3T3 cells (ab7179) were stained with ab8982 (1/50 and 1/20 respectively). The cells were fixed in 100% methanol for 6 minutes at -20°C. ab8982 clearly stains the nuclear envelope with very little background staining.

A : HeLa cells + ab8982 (1/50) (green)
B : HeLa cells counterstained with DAPI (blue)
C. 3T3 cells + ab8982 (1/20) (green)
D. 3T3 cells counterstained with DAPI (blue)

This image is courtesy of Marilena Ciciarello & Patrizia Lavia, University La Sapienza

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in epithelial and connective tissue cells.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Immunohistochemistry of MCF-7 cell culture showing nuclear lamina staining.

• Flow Cyt

Unknown

Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Overlay histogram showing HeLa cells stained with ab8982 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8982, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982)

Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.