重组Anti-LAMC2抗体[EPR23654-127] (ab274376)
![Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)](https://www.abcam.cn/ps/products/274/ab274376/Images/ab274376-1-anti-lamc2-antibody-epr23654127-immunocytochemistry-a4311.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23654-127] to LAMC2
- Suitable for: IP, Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LAMC2抗体[EPR23654-127]
参阅全部 LAMC2 一抗 -
描述
兔单克隆抗体[EPR23654-127] to LAMC2 -
宿主
Rabbit -
经测试应用
适用于: IP, Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 whole cell lysate. ICC/IF: A431 cells. Flow Cyt (intra): A549 cells. IP: A431 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23654-127 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274376于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30.
|
|
| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 80, 100, 140 kDa (predicted molecular weight: 131 kDa).
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
IP
1/30. |
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 80, 100, 140 kDa (predicted molecular weight: 131 kDa). |
|
ICC/IF
1/100. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells. -
组织特异性
The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli. -
疾病相关
Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. -
序列相似性
Contains 8 laminin EGF-like domains.
Contains 1 laminin IV type A domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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数据库链接
- Entrez Gene: 3918 Human
- Omim: 150292 Human
- SwissProt: Q13753 Human
- Unigene: 591484 Human
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别名
- 3918 antibody
- B2T antibody
- BM600 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling LAMC2 with ab274376 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line. Low expression control: A549 (PMID: 25591736). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)](https://www.abcam.cn/ps/products/274/ab274376/Images/ab274376-396974-anti-lamc2-antibody-epr23654127-western-blot-a4311.jpg)
Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)All lanes : Anti-LAMC2 antibody [EPR23654-127] (ab274376) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 131 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Bands of 140KDa and 80KDa respectively represent the full length and endogenously cleaved form.
The expression profile observed is consistent with what has been described in the literature (PMID: 25591736, 21829200).
Low expression: A549 (PMID:25591736) and WI-38 (PMID: 10964684)
Expsure time: 3 minutes
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![Flow Cytometry (Intracellular) - Anti-LAMC2 antibody [EPR23654-127] (ab274376)](https://www.abcam.cn/ps/products/274/ab274376/Images/ab274376-2-anti-lamc2-antibody-epr23654127-flow-cytometry-a5491.jpg)
Flow Cytometry (Intracellular) - Anti-LAMC2 antibody [EPR23654-127] (ab274376)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) (Left) A431 (Human epidermoid carcinoma epithelial cell) (Right) cells labelling LAMC2 with ab274376 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Low expression control: A549.
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![Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)](https://www.abcam.cn/ps/products/274/ab274376/Images/ab274376-3-anti-lamc2-antibody-epr23654127-immunoprecipitation-a4311.jpg)
Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)LAMC2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell), whole cell lysate with ab274376 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274376 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab274376 IP in A431 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274376 in A431 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
Band of 140KDa represents the full length form, Bands of 100KDa and 80KDa represent the endogenously cleaved form.
The expression profile observed is consistent with what has been described in the literature (PMID: 21829200).
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![Anti-LAMC2 antibody [EPR23654-127] (ab274376)](https://www.abcam.cn/ps/products/274/ab274376/Images/ab274376-5-benefits-of-recombinant-antibodies.png)
Anti-LAMC2 antibody [EPR23654-127] (ab274376)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab274376 被引用在 2 文献中.
- Zhao C et al. Long non-coding RNA BBOX1-antisense RNA 1 enhances cell proliferation and migration and suppresses apoptosis in oral squamous cell carcinoma via the miR-3940-3p/laminin subunit gamma 2 axis. Bioengineered 13:11138-11153 (2022). PubMed: 35506252
- You X et al. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway. Aging (Albany NY) 12:21837-21853 (2020). PubMed: 33170154