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AB124719

重组Anti-Lambda Light chain抗体[EPR5367-62]

Anti-Lambda Light chain antibody [EPR5367-62]

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(3 Publications)

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) is a rabbit monoclonal antibody detecting Lambda Light chain in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, ELISA. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

查看别名

Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1 : primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • ELISA

Lab

ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • WB

Lab

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • WB

Unknown

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution

Lane 1:

Human tonsil lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa,49 kDa,61 kDa

false

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)
  • WB

Lab

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution

All lanes:

Human thymus tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false

不同偶联物与剂型 (2)

  • HRP

    HRP Anti-Lambda Light chain antibody [EPR5367-62]

  • Carrier free

    Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5367-62

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ELISA, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified use at 1/50000 - 1/200000.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/500 - 1/1000.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/64000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.

What is the molecular weight of Lambda Light chain?
Anti-Lambda Light chain [EPR5367-62] (ab124719) specifically detects a band for Lambda Light chain (UniProt: P0CG04) at a molecular weight of 25kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5367-62] also available for your convenience: ab124719, Carrier free - ab185131, HRP - ab200966

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.
Biological function summary

Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.

Pathways

Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.

Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed : 24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
See full target information IGLV1-51
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33760139

2021

Expression of immunoglobulin G in human proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenling Deng,Ziyang Jing,Yanhong Guo,Junfan Ma,Huige Yan,Zhan Shi,Hui Deng,Yaoxian Liang,Song Wang,Zhuan Cui,Yuejuan Pan,Xiaoyan Qiu,Yue Wang

International journal of molecular medicine 41:3296-3306 PubMed29512722

2018

Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Jing,Hui Deng,Junfan Ma,Yanhong Guo,Yaoxian Liang,Rui Wu,Lata A,Zihan Geng,Xiaoyan Qiu,Yue Wang

Cancer letters 339:49-59 PubMed23887058

2013

Hsp70 inhibition induces myeloma cell death via the intracellular accumulation of immunoglobulin and the generation of proteotoxic stress.

Applications

WB

Species

Human

Lei Zhang,Jacqueline J L Fok,Fabio Mirabella,Lauren I Aronson,Rosemary A Fryer,Paul Workman,Gareth J Morgan,Faith E Davies
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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