Anti-Lambda Free Light Chain抗体[3D12] (ab1944)
Key features and details
- Mouse monoclonal [3D12] to Lambda Free Light Chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Lambda Free Light Chain抗体[3D12] -
描述
小鼠单克隆抗体[3D12] to Lambda Free Light Chain -
宿主
Mouse -
特异性
Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Lambda Free Light Chain.
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
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常规说明
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purity tested by electrophoresis. -
克隆
单克隆 -
克隆编号
3D12 -
骨髓瘤
Sp2/0 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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相关性
standard
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab1944 尚未被引用在任何文献中。