AB128864

重组Anti-LAMB3抗体[EPR7524]

Anti-LAMB3 antibody [EPR7524]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal LAMB3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

2 Images

Unknown

Western blot - Anti-LAMB3 antibody [EPR7524] (AB128864)

All lanes:

Western blot - Anti-LAMB3 antibody [EPR7524] (ab128864) at 1/1000 dilution

Lane 1:

HACAT cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 130 kDa

Observed band size: 140 kDa

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OI-RD Scanning - Anti-LAMB3 antibody [EPR7524] (AB128864)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LAMB3 antibody [EPR7524] (AB128864)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-LAMB3 antibody [EPR7524] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7524

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit beta-3 (LAMB3) is a protein that is part of the laminin family commonly referred to as Laminin-332 or Laminin-5. It weighs approximately 117 kDa. LAMB3 is expressed in the epithelial basement membranes of various tissues including skin and mucous membranes. The protein forms a network essential for maintaining the structural integrity of these membranes playing a mechanical role in cellular adhesion and migration.

Biological function summary

LAMB3 functions within a heterotrimeric complex that includes Laminin-3 (alpha subunit) and Laminin-3 (gamma subunit). These interactions lead to the formation of Laminin-332 which serves as a backbone for cell attachment and spreading. It is important in processes such as epithelial cell proliferation and wound healing. By stabilizing attachments between the basement membrane and epithelial cells LAMB3 supports tissue integrity and repair mechanisms.

Pathways

LAMB3 plays a role in both the integrin signaling and epidermal growth factor receptor (EGFR) pathways. It connects with proteins like the integrin alpha-6 beta-4 complex facilitating cell adhesion and communication with the extracellular matrix. In the EGFR pathway LAMB3's interactions enhance signaling that promotes cellular proliferation and survival playing a part in skin development and maintenance.

Mutations or dysregulations in LAMB3 relate notably to junctional epidermolysis bullosa and certain cancers like squamous cell carcinoma. In junctional epidermolysis bullosa the protein loses its adhesive function affecting the epithelial junctions and causing severe skin blistering. LAMB3 also associates with integrin proteins in cancer contributing to tumor progression and metastasis when dysfunctional affecting cell adhesion and integrity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 23:4495-505 PubMed23051733

2012

Establishing epithelial glandular polarity: interlinked roles for ARF6, Rac1, and the matrix microenvironment.

Applications

Unspecified application

Species

Dog

Christine L Monteleon,Alanna Sedgwick,Alyssa Hartsell,Michael Dai,Catherine Whittington,Sherry Voytik-Harbin,Crislyn D'Souza-Schorey

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-LAMB3抗体[EPR7524]