重组Anti-LAG-3抗体[EPR4392(2)] - C-terminal (ab180187)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)](https://www.abcam.cn/ps/products/180/ab180187/Images/ab180187-208825-anti-lymphocyte-activation-gene-3-antibody-epr43922-c-terminal-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4392(2)] to LAG-3 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LAG-3抗体[EPR4392(2)] - C-terminal
参阅全部 LAG-3 一抗 -
描述
兔单克隆抗体[EPR4392(2)] to LAG-3 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details
不适用于: ICC/IF,IP or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LAG-3 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P18627 -
阳性对照
- Human Hodgkins lymphoma and Human tonsil tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4392(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
- Alexa Fluor® 488 Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab225277)
- Alexa Fluor® 647 Anti-LAG-3 antibody [EPR4392(2)] (ab225278)
- Anti-LAG-3 antibody [EPR4392(2)] - Low endotoxin, Azide free (ab282642)
- APC Anti-LAG-3 antibody [EPR4392(2)] – C–terminal (ab319324)
- PE Anti-LAG-3 antibody [EPR4392(2)] – C–terminal (ab319461)
- Alexa Fluor® 594 Anti-LAG-3 antibody [EPR4392(2)] – C–terminal (ab319816)
- Alexa Fluor® 555 Anti-LAG-3 antibody [EPR4392(2)] – C–terminal (ab319957)
- Alexa Fluor® 750 Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab321228)
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
组织特异性
On cell surface of activated NK and T-lymphocytes. -
序列相似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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别名
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LAG-3 with ab180187 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)](https://www.abcam.cn/ps/products/180/ab180187/Images/ab180187-208824-anti-lymphocyte-activation-gene-3-antibody-epr43922-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)Immunohistochemical analysis of paraffin embedded Human Hodgkins lymphoma tissue labeling LAG-3 with ab180187 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)](https://www.abcam.cn/ps/products/180/ab180187/Images/ab180187-7-benefits-of-recombinant-antibodies.png)
Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab180187 被引用在 15 文献中.
- Zaitsu S et al. Lymphocyte-activation gene 3 protein expression in tumor-infiltrating lymphocytes is associated with a poor prognosis of ovarian clear cell carcinoma. J Ovarian Res 16:93 (2023). PubMed: 37179337
- Luke JJ et al. The PD-1- and LAG-3-targeting bispecific molecule tebotelimab in solid tumors and hematologic cancers: a phase 1 trial. Nat Med 29:2814-2824 (2023). PubMed: 37857711
- Zacharias M et al. Host and microbiome features of secondary infections in lethal covid-19. iScience 25:104926 (2022). PubMed: 35992303
- Luo F et al. Correction to: Lymphocyte activating gene 3 protein expression in nasopharyngeal carcinoma is correlated with programmed cell death-1 and programmed cell death ligand-1, tumor-infiltrating lymphocytes. Cancer Cell Int 22:46 (2022). PubMed: 35093085
- Machiraju D et al. Soluble immune checkpoints and T-cell subsets in blood as biomarkers for resistance to immunotherapy in melanoma patients. Oncoimmunology 10:1926762 (2021). ELISA, IHC-P ; Human . PubMed: 34104542