重组Anti-LAG-3抗体[BLR027F] - BSA free (ab272078)
![Western blot - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)](https://www.abcam.cn/ps/products/272/ab272078/Images/ab272078-468313-ab243884-anti-lag-3-beta-antibody-western-blot.jpg "Western blot - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR027F] to LAG-3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LAG-3抗体[BLR027F] - BSA free
参阅全部 LAG-3 一抗 -
描述
兔单克隆抗体[BLR027F] to LAG-3 - BSA free -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
Database link: P18627 -
阳性对照
- WB: HDLM-2 whole cell lysate. IP: HDLM-2 whole cell lysate. ICC: HDLM-2 cells. IHC-P: Human tonsil tissue.
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR027F -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
组织特异性
On cell surface of activated NK and T-lymphocytes. -
序列相似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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别名
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
图片
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Western blot - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)All lanes : Anti-LAG-3 antibody [BLR027F] (ab243884) at 1/1000 dilution
Lane 1 : HDLM-2 whole cell lysate
Lane 2 : RPMI 8226 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : MJ whole cell lysate
Lane 5 : HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 30 secondsDetection: Chemiluminescence.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).
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![Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)](https://www.abcam.cn/ps/products/272/ab272078/Images/ab272078-1-ab243884334328antilymphocyteactivationgene3antibodyblr027fimmunoprecipitation.jpg)
Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.
Lane 1: ab243884 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).
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![Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)](https://www.abcam.cn/ps/products/272/ab272078/Images/ab272078-2-ab243884334326antilymphocyteactivationgene3antibodyblr027fimmunocytochemistry.jpg)
Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)](https://www.abcam.cn/ps/products/272/ab272078/Images/ab272078-3-ab243884334325antilymphocyteactivationgene3antibodyblr027fimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).
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![Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)](https://www.abcam.cn/ps/products/272/ab272078/Images/ab272078-4-benefits-of-recombinant-antibodies.png)
Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272078 尚未被引用在任何文献中。