重组Anti-Lactoferrin抗体[EPR4337] (ab109216)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4337] to Lactoferrin
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lactoferrin抗体[EPR4337]
参阅全部 Lactoferrin 一抗 -
描述
兔单克隆抗体[EPR4337] to Lactoferrin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: PBMC, HeLa, fetal kidney and Human milk lysates ICC/IF: HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4337 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Lactoferrin antibody [EPR4337] - BSA and Azide free (ab247794)
- PE Anti-Lactoferrin antibody [EPR4337] (ab318367)
- APC Anti-Lactoferrin antibody [EPR4337] (ab318470)
- Alexa Fluor® 488 Anti-Lactoferrin antibody [EPR4337] (ab318573)
- Alexa Fluor® 647 Anti-Lactoferrin antibody [EPR4337] (ab318676)
- Alexa Fluor® 594 Anti-Lactoferrin antibody [EPR4337] (ab318779)
- Alexa Fluor® 555 Anti-Lactoferrin antibody [EPR4337] (ab318879)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109216于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 78 kDa).
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ICC/IF |
1/100 - 1/250.
|
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 78 kDa). |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
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别名
- GIG12 antibody
- Growth inhibiting protein 12 antibody
- HLF2 antibody
see all
图片
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All lanes : Anti-Lactoferrin antibody [EPR4337] (ab109216) at 1/1000 dilution
Lane 1 : PBMC lysate
Lane 2 : HeLa lysate
Lane 3 : Fetal kidney lysate
Lane 4 : Human milk lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted? -
ab109216, at a 1/100 dilution, staining Lactoferrin in HepG2 cells by Immunocytochemistry/ Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109216 被引用在 1 文献中.
- Wu CX & Liu ZF Proteomic Profiling of Sweat Exosome Suggests its Involvement in Skin Immunity. J Invest Dermatol 138:89-97 (2018). PubMed: 28899687