Anti-Lactoferrin抗体(ab77548)
Key features and details
- Goat polyclonal to Lactoferrin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Lactoferrin抗体
参阅全部 Lactoferrin 一抗 -
描述
山羊多克隆抗体to Lactoferrin -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
C-ENYKSQQSSDPDP
, corresponding to internal sequence amino acids 432-444 of Human Lactoferrin (NP_002334.2). -
阳性对照
- WB: Human Peripheral Blood Mononucleocyte lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77548 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77548于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa.
A 1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa. A 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
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别名
- GIG12 antibody
- Growth inhibiting protein 12 antibody
- HLF2 antibody
see all
图片
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Western blot - Anti-Lactoferrin antibody (ab77548)Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µg
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab77548 被引用在 1 文献中.
- Alhalwani AY et al. Development of a sandwich ELISA with potential for selective quantification of human lactoferrin protein nitrated through disease or environmental exposure. Anal Bioanal Chem 410:1389-1396 (2018). PubMed: 29214534
