重组Anti-LACTB抗体[EPR8502] (ab131171)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8502] to LACTB
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LACTB抗体[EPR8502]
参阅全部 LACTB 一抗 -
描述
兔单克隆抗体[EPR8502] to LACTB -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Skeletal Muscle (Human) Whole Cell Lysate - fetal normal tissue (ab29331) can be used as a positive control in WB. HepG2, HeLa, Human fetal heart and Human fetal muscle lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.27 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8502 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa). |
靶标
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相关性
LACTB is a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). It has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. It is expressed predominantly in skeletal muscle. There are two named isoforms. -
细胞定位
Mitochondrion -
数据库链接
- Entrez Gene: 114294 Human
- Entrez Gene: 80907 Mouse
- Entrez Gene: 300803 Rat
- Omim: 608440 Human
- SwissProt: P83111 Human
- SwissProt: Q9EP89 Mouse
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别名
- FLJ14902 antibody
- G24 antibody
- Lactamase, beta antibody
see all
图片
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All lanes : Anti-LACTB antibody [EPR8502] (ab131171) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131171 被引用在 1 文献中.
- Tan P et al. Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR-373-5p. Cancer Sci 114:1396-1409 (2023). PubMed: 36562402