重组Anti-L3MBTL3抗体[EPR11854] (ab175232)
![Western blot - Anti-L3MBTL3 antibody [EPR11854] (ab175232)](https://www.abcam.cn/ps/products/175/ab175232/Images/ab175232-412562-anti-l3mbtl3-antibody-epr11854-western-blot-human-knockout-lysate.jpg "Western blot - Anti-L3MBTL3 antibody [EPR11854] (ab175232)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11854] to L3MBTL3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-L3MBTL3抗体[EPR11854]
参阅全部 L3MBTL3 一抗 -
描述
兔单克隆抗体[EPR11854] to L3MBTL3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment within Human L3MBTL3. The exact sequence is proprietary.
Database link: Q96JM7 -
阳性对照
- WB: HeLa, 293T and Human fetal brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11854 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175232于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa. |
靶标
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功能
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells. -
序列相似性
Contains 3 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 84456 Human
- SwissProt: Q96JM7 Human
- Unigene: 658051 Human
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别名
- L3MBTL3 antibody
- H-l(3)mbt-like protein 3 antibody
- Hl(3)mbt like protein antibody
see all
图片
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Western blot - Anti-L3MBTL3 antibody [EPR11854] (ab175232)All lanes : Anti-L3MBTL3 antibody [EPR11854] (ab175232) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : L3MBTL3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaLanes 1-4: Merged signal (red and green). Green - ab175232 observed at 88 kDa. Red - loading control ab8245 observed at 36 kDa.
ab175232 Anti-L3MBTL3 antibody [EPR11854] was shown to specifically react with L3MBTL3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266172 (knockout cell lysate ab258489) was used. Wild-type and L3MBTL3 knockout samples were subjected to SDS-PAGE. ab175232 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-L3MBTL3 antibody [EPR11854] (ab175232)](https://www.abcam.cn/ps/products/175/ab175232/Images/ab175232-201599-anti-l3mbtl3-antibody-epr11854-western-blot.jpg)
Western blot - Anti-L3MBTL3 antibody [EPR11854] (ab175232)All lanes : Anti-L3MBTL3 antibody [EPR11854] (ab175232) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 88 kDa -
![Anti-L3MBTL3 antibody [EPR11854] (ab175232)](https://www.abcam.cn/ps/products/175/ab175232/Images/ab175232-3-benefits-of-recombinant-antibodies.png)
Anti-L3MBTL3 antibody [EPR11854] (ab175232)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab175232 尚未被引用在任何文献中。