Anti-L Glutamate抗体(ab9440)
Key features and details
- Rabbit polyclonal to L Glutamate
- Suitable for: IHC-FoFr, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-L Glutamate抗体
参阅全部 L Glutamate 一抗 -
描述
兔多克隆抗体to L Glutamate -
宿主
Rabbit -
特异性
The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue). -
经测试应用
适用于: IHC-FoFr, IHC-Frmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to L Glutamate (Glutaraldehyde).
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常规说明
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: Phosphate Buffer, 1% Goat Serum -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | (1) |
Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-FoFr
Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. |
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IHC-Fr
Use at an assay dependent concentration. |
靶标
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相关性
L Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System. -
别名
- Glutamate antibody
- L glutamate antibody
图片
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Immunohistochemistry (Frozen sections) - Anti-L Glutamate antibody (ab9440)This image is courtesy of an Abreview submitted by Patricia Bogdanov
ab9440 staining L Gluramate in Mouse retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.025% TBS-Triton X-100 and blocked with 5% serum for 1 hour. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab9440 被引用在 13 文献中.
- Otubo A et al. Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at -25 °C for Several Years. Int J Mol Sci 22:N/A (2021). PubMed: 34502087
- Yu XJ et al. Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension. Front Neurosci 15:805070 (2021). PubMed: 34938159
- Shivashankar G et al. Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions. Mol Vis 26:277-290 (2020). PubMed: 32300272
- Chang LY et al. Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus. Front Neurosci 14:161 (2020). PubMed: 32256305
- Tsai HY et al. Loss of the Drosophila branched-chain a-ketoacid dehydrogenase complex results in neuronal dysfunction. Dis Model Mech 13:N/A (2020). PubMed: 32680850