Anti-Kv4.2/KCND2抗体[EP982Y] (ab46797)
![Western blot - Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797)](https://www.abcam.cn/ps/products/46/ab46797/Images/ab46797-59031-anti-kv42-antibody-ep982y-western-blot.jpg "Western blot - Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797)")
Key features and details
- Rabbit monoclonal [EP982Y] to Kv4.2/KCND2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-Kv4.2/KCND2抗体[EP982Y]
参阅全部 Kv4.2/KCND2 一抗 -
描述
兔单克隆抗体[EP982Y] to Kv4.2/KCND2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human brain lysate. IHC-P: Human cerebrum tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP982Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46797于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 80 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 80 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. -
组织特异性
Highly expressed throughout the brain. Expression is very low or absent in other tissues. -
序列相似性
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. -
结构域
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
翻译后修饰
Phosphorylated on serine and threonine residues. -
细胞定位
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 3751 Human
- Omim: 605410 Human
- SwissProt: Q9NZV8 Human
- Unigene: 654739 Human
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别名
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
see all
图片
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Western blot - Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797)Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797) at 1/1000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797)](https://www.abcam.cn/ps/products/46/ab46797/Images/ab46797-3-anti-kv4-2-kcnd2-antibody-Immunohistochemistry-cerebrumhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue sections labeling Kv4.2/KCND2 with purified ab46797 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab46797 被引用在 2 文献中.
- Cacace R et al. Loss of DPP6 in neurodegenerative dementia: a genetic player in the dysfunction of neuronal excitability. Acta Neuropathol 137:901-918 (2019). WB ; Human . PubMed: 30874922
- Aronica E et al. Expression and localization of voltage dependent potassium channel Kv4.2 in epilepsy associated focal lesions. Neurobiol Dis 36:81-95 (2009). PubMed: 19596445