重组Anti-Ku80抗体[EPR3467] (ab79391)
![Western blot - Anti-Ku80 antibody [EPR3467] (ab79391)](https://www.abcam.cn/ps/products/79/ab79391/Images/ab79391-94940-anti-ku80-antibody-epr3467-western-blot.jpg "Western blot - Anti-Ku80 antibody [EPR3467] (ab79391)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3467] to Ku80
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ku80抗体[EPR3467]
参阅全部 Ku80 一抗 -
描述
兔单克隆抗体[EPR3467] to Ku80 -
宿主
Rabbit -
特异性
The immunogen for ab79391 has 78.6% homology with mouse and rat ku80, therefore we do not guarantee reactivity in these species. -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Ku80 aa 50-150. The exact sequence is proprietary.
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阳性对照
- A549 (treated with FBS), HeLa, U937 and HepG2 cell lysates; human breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3467 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79391于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
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| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
序列相似性
Belongs to the ku80 family.
Contains 1 Ku domain. -
发展阶段
Expression increases during promyelocyte differentiation. -
结构域
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
翻译后修饰
Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Sumoylated. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 7520 Human
- Omim: 194364 Human
- SwissProt: P13010 Human
- Unigene: 388739 Human
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别名
- 86 kDa subunit of Ku antigen antibody
- ATP dependent DNA helicase 2 subunit 2 antibody
- ATP dependent DNA helicase II 80 kDa subunit antibody
see all
图片
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Western blot - Anti-Ku80 antibody [EPR3467] (ab79391)All lanes : Anti-Ku80 antibody [EPR3467] (ab79391) at 1/2000 dilution
Lane 1 : A549 cell lysate, treated with FBS
Lane 2 : HeLa cell lysate
Lane 3 : U937 (Human histiocytic lymphoma cell line) cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa -
![Flow Cytometry (Intracellular) - Anti-Ku80 antibody [EPR3467] (ab79391)](https://www.abcam.cn/ps/products/79/ab79391/Images/ab79391-195328-anti-ku80-antibody-epr3467-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ku80 antibody [EPR3467] (ab79391)Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79391, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [EPR3467] (ab79391)](https://www.abcam.cn/ps/products/79/ab79391/Images/ab79391-94939-anti-ku80-antibody-epr3467-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [EPR3467] (ab79391)ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Ku80 antibody [EPR3467] (ab79391)](https://www.abcam.cn/ps/products/79/ab79391/Images/ab79391-5-benefits-of-recombinant-antibodies.png)
Anti-Ku80 antibody [EPR3467] (ab79391)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab79391 被引用在 3 文献中.
- Petrigliano FA et al. Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. NPJ Regen Med 6:77 (2021). PubMed: 34815400
- Unoki M et al. CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J Clin Invest 129:78-92 (2019). PubMed: 30307408
- Dumbovic G et al. A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer. Nucleic Acids Res 46:5504-5524 (2018). PubMed: 29912433