Anti-Ku70 (phospho S5)抗体(ab61783)
Key features and details
- Rabbit polyclonal to Ku70 (phospho S5)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Ku70 (phospho S5)抗体
参阅全部 Ku70 一抗 -
描述
兔多克隆抗体to Ku70 (phospho S5) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human Ku70 aa 1-100 (phospho S5).
Database link: P12956 -
阳性对照
- Extracts from HeLa cells (WB) Human lung carcinoma tissue (IHC)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 70 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 70 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
序列相似性
Belongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain. -
发展阶段
Expression does not increase during promyelocyte differentiation. -
翻译后修饰
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 2547 Human
- Entrez Gene: 14375 Mouse
- Omim: 152690 Human
- SwissProt: P12956 Human
- SwissProt: P23475 Mouse
- Unigene: 292493 Human
- Unigene: 288809 Mouse
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别名
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 (phospho S5) antibody (ab61783)Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue using ab61783 at 1/50 dilution in the presence and abscence of immunizing peptide antibody.
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Western blot - Anti-Ku70 (phospho S5) antibody (ab61783)All lanes : Anti-Ku70 (phospho S5) antibody (ab61783) at 1/500 dilution
Lane 1 : extracts from HeLa cells with no immunizing peptide
Lane 2 : extracts from HeLa cells with immunizing peptide
Predicted band size: 70 kDa
Observed band size: 70 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab61783 被引用在 4 文献中.
- Timm S et al. Clustered DNA damage concentrated in particle trajectories causes persistent large-scale rearrangements in chromatin architecture. Radiother Oncol N/A:N/A (2018). PubMed: 30049456
- Natale F et al. Identification of the elementary structural units of the DNA damage response. Nat Commun 8:15760 (2017). PubMed: 28604675
- Schuler N et al. DNA-damage foci to detect and characterize DNA repair alterations in children treated for pediatric malignancies. PLoS One 9:e91319 (2014). IHC ; Human . PubMed: 24637877
- Schuler N & Rübe CE Accumulation of DNA damage-induced chromatin alterations in tissue-specific stem cells: the driving force of aging? PLoS One 8:e63932 (2013). Flow Cyt ; Mouse . PubMed: 23691119