Anti-KSR1 (phospho S404)抗体[EP2358Y] (ab76248)
![Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.cn/ps/products/76/ab76248/Images/ab76248.JPG "Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)")
Key features and details
- Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-KSR1 (phospho S404)抗体[EP2358Y]
参阅全部 KSR1 一抗 -
描述
兔单克隆抗体[EP2358Y] to KSR1 (phospho S404) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysates, untreated or treated with Calyculin A ICC/IF: Sh-SY-5Y cells
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2358Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/250. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
靶标
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功能
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. -
细胞定位
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. - Information by UniProt
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数据库链接
- Entrez Gene: 8844 Human
- Omim: 601132 Human
- SwissProt: Q8IVT5 Human
- Unigene: 133534 Human
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别名
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
see all
图片
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Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.cn/ps/products/76/ab76248/Images/ab76248 IF.JPG)
Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab76248 尚未被引用在任何文献中。