AB76248

Anti-KSR1 (phospho S404)抗体[EP2358Y]

Anti-KSR1 (phospho S404) antibody [EP2358Y]

RabMAb
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(1 Publication)

Rabbit Monoclonal KSR1 phospho S404 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

KSR, KSR1, Kinase suppressor of Ras 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KSR1 (phospho S404) antibody [EP2358Y] (AB76248)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KSR1 (phospho S404) antibody [EP2358Y] (AB76248)

Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.

Unknown

Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (AB76248)

All lanes:

Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution

Lane 1:

HeLa cell lysate (untreated) at 10 µg

Lane 2:

HeLa cell lysate, treated with Calyculin A at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labelled IgG at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 110 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-KSR1 (phospho S404) antibody [EP2358Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2358Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.

Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).

See full target information KSR1 phospho S404

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70098 PubMed39491498

2024

Fasudil Alleviates Postoperative Neurocognitive Disorders in Mice by Downregulating the Surface Expression of α5GABAAR in Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Dong,Zhun Wang,Lixuan Li,Mengxue Zhang,Sixuan Wang,Yuan Luo,Ying Dong,Xiaokun Wang,Yongan Wang,Kaiyuan Wang,Yiqing Yin

View all publications

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Anti-KSR1 (phospho S404)抗体[EP2358Y]