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AB278551

重组Anti-KSR1 (phospho S392)抗体[KSR1S392-3A4]

Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

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Rabbit Recombinant Monoclonal KSR1 phospho S392 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KSR1 pS392.

查看别名

KSR, KSR1, Kinase suppressor of Ras 1

3 Images
Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells secondary antibody only negative control (blue) treated with imatinib (red) treated with PV (green) treated with PV + blocked with non-phospho peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using ab278551 at 0.05 μg/ml.

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using ab278551 at 0.1 μg/ml.

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278551)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only (blue) or 0.1 μg/ml of isotype control (orange) or ab278551 (green).

不同偶联物与剂型 (2)

  • 519 FITC

    FITC Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

  • 578 PE

    PE Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

KSR1S392-3A4

亚型

IgG

轻链类型

kappa

不含载体蛋白

No

反应种属

Human, Mouse

应用

Flow Cyt

applications

免疫原

Synthetic Peptide within Human KSR1 pS392. The exact immunogen used to generate this antibody is proprietary information.

Q8IVT5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
See full target information KSR1 pS392
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搭配使用产品

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