Anti-KRT81抗体[3B10-5B10] (ab55407)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (ab55407)](https://www.abcam.cn/ps/products/55/ab55407/Images/ab55407-68658-anti-krt81-antibody-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (ab55407)")
Key features and details
- Mouse monoclonal [3B10-5B10] to KRT81
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-KRT81抗体[3B10-5B10]
参阅全部 KRT81 一抗 -
描述
小鼠单克隆抗体[3B10-5B10] to KRT81 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein, corresponding to amino acids 304-505 of Human KRT81.
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常规说明
This product was changed from ascites to tissue culture supernatant on 27/June/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
3B10-5B10 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55407于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ICC/IF | ||
| IHC-P |
| 说明 |
|---|
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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组织特异性
Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue. -
疾病相关
Defects in KRT81 are a cause of monilethrix (MLTRX) [MIM:158000]. Monilethrix is an autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected. -
序列相似性
Belongs to the intermediate filament family. - Information by UniProt
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数据库链接
- Entrez Gene: 3887 Human
- Omim: 602153 Human
- SwissProt: Q14533 Human
- Unigene: 658118 Human
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别名
- 1 antibody
- basic antibody
- ghHb 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT81 antibody [3B10-5B10] (ab55407)
KRT81 antibody (ab55407) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus. This was producted using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-KRT81 antibody [3B10-5B10] (ab55407)](https://www.abcam.cn/ps/products/55/ab55407/Images/ab55407-68659-anti-krt81-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KRT81 antibody [3B10-5B10] (ab55407)KRT81 antibody (ab55407) used in immunofluorescence at 10ug/ml on HeLa cells. This was producted using the ascites version of the product.
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![Western blot - Anti-KRT81 antibody [3B10-5B10] (ab55407)](https://www.abcam.cn/ps/products/55/ab55407/Images/ab55407-68657-anti-krt81-antibody-western-blot.jpg)
Western blot - Anti-KRT81 antibody [3B10-5B10] (ab55407)KRT81 antibody (ab55407) at 1ug/lane + HepG2 cell lysate at 25ug/lane. This was producted using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab55407 被引用在 2 文献中.
- Wang X et al. Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells. Mol Cancer 21:133 (2022). PubMed: 35733218
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873