AB6039

Anti-KPNA4抗体

Name: KPNA4抗体 (ab6039)| Abcam中文官网
Brand: Abcam Limited
SKU: AB6039
Price: 4606 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-KPNA4 antibody

(3 Reviews)

(20 Publications)

Goat Polyclonal KPNA4 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA4 aa 500 to C-terminus.

查看别名

QIP1, KPNA4, Importin subunit alpha-3, Importin alpha Q1, Karyopherin subunit alpha-4, Qip1

1 Images

Supplier Data

Western blot - Anti-KPNA4 antibody (AB6039)

Lysates in RIPA buffer.

Detected by chemiluminescence.

Lanes 1 - 2:

Western blot - Anti-KPNA4 antibody (ab6039) at 0.1 µg/mL

Lane 3:

Western blot - Anti-KPNA4 antibody (ab6039) at 0.3 µg/mL

Lane 4:

Western blot - Anti-KPNA4 antibody (ab6039) at 0.03 µg/mL

Lane 1:

Human ovary lysate at 35 µg

Lane 2:

Mouse testis lysate at 35 µg

Lane 3:

Mouse ovary lysate at 35 µg

Lane 4:

Rat testis lysate at 35 µg

Predicted band size: 57 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

Synthetic Peptide within Human KPNA4 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00629

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

GenBank Accession Number – NP_002259.

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KPNA4 also known as importin alpha 4 or karyopherin subunit alpha 4 is a protein involved in nuclear transport mechanisms. It has a molecular weight of approximately 58 kDa. KPNA4 finds expression in various tissues including the brain immune cells and reproductive organs. This protein belongs to the family of karyopherins which mediates the import of proteins into the nucleus by recognizing nuclear localization signals.

Biological function summary

KPNA4 plays a role in transporting proteins into the nucleus as part of the importin complex. This complex forms through the association of KPNA4 with importin beta facilitating the translocation of cargo proteins through the nuclear pore complex. This translocation process is essential for regulating gene expression and cellular functions as proteins must reach the cell nucleus to execute their roles. KPNA4 interacts with various cargo proteins allowing them to partake in nuclear activities essential for cellular activity and response.

Pathways

KPNA4 participates in the nuclear import pathway closely linking with the Ran GTPase cycle. This pathway is important for transporting macromolecules between the cytoplasm and nucleus. KPNA4's action complements the function of the importin beta family where it co-transports with proteins like Ran and importin beta ensuring effective and specific nuclear import of karyophilic proteins. The presence of KPNA4 in these pathways highlights its importance in cellular regulation and response paths.

KPNA4 shows connections to neurodegenerative diseases and certain cancers suggesting its potential role in these conditions. In neurodegenerative diseases its association with proteins involved in intracellular transport could imply a role in pathological processes where nuclear-cytoplasmic trafficking is disrupted. Furthermore KPNA4's interaction with proteins like importin beta in cancer could influence tumor progression through altered nuclear transport impacting gene expression and cell cycle regulation. Recognizing these associations might provide insights for therapeutic targeting or diagnostic markers in related conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 10567565, PubMed : 20818336, PubMed : 28760339, PubMed : 29042532, PubMed : 38512451). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 20818336, PubMed : 28760339, PubMed : 29042532, PubMed : 38512451). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 20818336, PubMed : 28760339, PubMed : 29042532, PubMed : 38512451). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed : 20818336, PubMed : 28760339, PubMed : 29042532, PubMed : 38512451). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 20818336, PubMed : 28760339, PubMed : 29042532, PubMed : 38512451). Mediates nuclear import of AARS1, MRTFA and RANBP3 (PubMed : 10567565, PubMed : 20818336, PubMed : 28760339, PubMed : 38512451).. (Microbial infection) In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

See full target information KPNA4

style

left-column

文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:2103 PubMed33833234

2021

Exploiting pyocyanin to treat mitochondrial disease due to respiratory complex III dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Peruzzo,Samantha Corrà,Roberto Costa,Michele Brischigliaro,Tatiana Varanita,Lucia Biasutto,Chiara Rampazzo,Daniele Ghezzi,Luigi Leanza,Mario Zoratti,Massimo Zeviani,Cristiano De Pittà,Carlo Viscomi,Rodolfo Costa,Ildikò Szabò

Virulence 12:754-765 PubMed33616472

2021

Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Xie,Weikang Wang,Luyuan Li,Qiuqi Kan,Hui Fu,Tuoyu Geng,Tuofan Li,Zhimin Wan,Wei Gao,Hongxia Shao,Aijian Qin,Jianqiang Ye

Molecular medicine reports 23: PubMed33537819

2021

CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia‑induced damage.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Liu,Hongchao Zhao,Qingqing Ding,Haohao Li,Tao Liu,Hongwei Yang,Yuanhua Liu

Cancer cell international 20:568 PubMed33327962

2020

LncRNA ST7-AS1, by regulating miR-181b-5p/KPNA4 axis, promotes the malignancy of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rong-Hang Hu,Zi-Teng Zhang,Hai-Xiang Wei,Lu Ning,Jiang-Shan Ai,Wen-Hui Li,Heng Zhang,Shao-Qiang Wang

Cell reports 31:107549 PubMed32320654

2020

Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Swantje Thiele,Stephanie Stanelle-Bertram,Sebastian Beck,Nancy Mounogou Kouassi,Martin Zickler,Martin Müller,Berfin Tuku,Patricia Resa-Infante,Debby van Riel,Malik Alawi,Thomas Günther,Franziska Rother,Stefanie Hügel,Susanne Reimering,Alice McHardy,Adam Grundhoff,Wolfram Brune,Albert Osterhaus,Michael Bader,Enno Hartmann,Gülsah Gabriel

Cardiovascular diabetology 19:20 PubMed32066445

2020

Vascular miR-181b controls tissue factor-dependent thrombogenicity and inflammation in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Witkowski,Mario Witkowski,Mona Saffarzadeh,Julian Friebel,Termeh Tabaraie,Loc Ta Bao,Aritra Chakraborty,Andrea Dörner,Bernd Stratmann,Diethelm Tschoepe,Samantha J Winter,Andreas Krueger,Wolfram Ruf,Ulf Landmesser,Ursula Rauch

Journal of cell science 132: PubMed31434716

2019

Phosphorylation of importin-α1 by CDK1-cyclin B1 controls mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Li Guo,Khamsah Suryati Mohd,He Ren,Guangwei Xin,Qing Jiang,Paul R Clarke,Chuanmao Zhang

Experimental and therapeutic medicine 15:338-344 PubMed29250154

2017

MicroRNA-24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin-α3 and regulating inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Zheng,Yongxing Li,Gang Liu,Xiangqian Qi,Xufen Cao

Scientific reports 7:43323 PubMed28240251

2017

Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin α proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew T Major,Yoichi Miyamoto,Camden Y Lo,David A Jans,Kate L Loveland

Biochemical and biophysical research communications 479:429-433 PubMed27622322

2016

Nucleocytoplasmic trafficking of Nipah virus W protein involves multiple discrete interactions with the nuclear import and export machinery.

Applications

Species

Human

Michelle D Audsley,David A Jans,Gregory W Moseley

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KPNA4抗体

Anti-KPNA4 antibody

Western blot - Anti-KPNA4 antibody (AB6039)