Anti-KPC2抗体- C-terminal (ab177519)
Key features and details
- Rabbit polyclonal to KPC2 - C-terminal
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KPC2抗体- C-terminal
参阅全部 KPC2 一抗 -
描述
兔多克隆抗体to KPC2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide within Human KPC2 aa 325-375 (C terminal). The exact sequence is proprietary. NP_057256.2
Sequence:LLGDRK PSPEELDKGI DPDSPLFQAI LDNPVVQLGL TNPKTLLAFE DMLEN
Database link: Q9BSL1 -
阳性对照
- WB: 293T, HeLa, Jurkat and TCMK1 whole cell lysates. IP: HEK-293T cell lysates. IHC-P: Human colon carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177519于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
靶标
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相关性
KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 746249 Chimpanzee
- Entrez Gene: 491256 Dog
- Entrez Gene: 101139469 Gorilla
- Entrez Gene: 100722364 Guinea pig
- Entrez Gene: 10422 Human
- Entrez Gene: 98766 Mouse
- Entrez Gene: 100441464 Orangutan
- Entrez Gene: 362087 Rat
see all -
别名
- 1110033G07Rik antibody
- AA407978 antibody
- E3 ubiquitin-protein ligase subunit KPC2 antibody
see all
图片
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Western blot - Anti-KPC2 antibody - C-terminal (ab177519)All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody - C-terminal (ab177519)Immunohistochemical analysis of human colon carcinoma tissue labeling KPC2 with ab177519 at a 1/1000 dilution.
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Immunoprecipitation - Anti-KPC2 antibody - C-terminal (ab177519)All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 1 µg/ml
Lane 1 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using ab177519 at 6 µg/mg lysate.
Lane 2 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using Control IgG at 6 µg/mg lysate.
Secondary
All lanes : Anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab177519 被引用在 2 文献中.
- Guo M et al. Targeting RFWD2 as an Effective Strategy to Inhibit Cellular Proliferation and Overcome Drug Resistance to Proteasome Inhibitor in Multiple Myeloma. Front Cell Dev Biol 9:675939 (2021). PubMed: 33968945
- Guégan JP et al. CD95/Fas suppresses NF-κB activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism. iScience 24:103538 (2021). PubMed: 34917906