JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283270

重组Anti-KMT6 /EZH2抗体[11/EZH2]

Anti-KMT6 /EZH2 antibody [11/EZH2]

  • BOND RX™ Validated
  • Recombinant
  • KO Validated
  • 了解详情

Be the first to review this product! Submit a review

|

(14 Publications)

Mouse Recombinant Monoclonal KMT6 / EZH2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

查看别名

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling KMT6/EZH2 with ab283270 at 1/100 dilution (10.9 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in human ovarian cancer. The section was incubated with ab283270 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling KMT6/EZH2 with ab283270 at 1/100 (10.9 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in mouse colon. The section was incubated with ab283270 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KMT6/EZH2 with ab283270 at 1/100 dilution (10.9 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in rat colon. The section was incubated with ab283270 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)
  • WB

Supplier Data

Western blot - Anti-KMT6 /EZH2 antibody [11/EZH2] (AB283270)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lanes 1 - 4 : Merged signal (red and green). Green - ab283270 observed at 911 kDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

Lanes 1-2 : ab283270 Anti-EZH2 antibody was shown to react with EZH2 in HAP1 cells in Western blot. Loss of signal was observed when EZH2 knockout sample was used. Wild-type and EZH2 knockout samples were subjected to SDS-PAGE.

ab283270 and Anti-GAPDH antibody [EPR16891] (ab181602) were incubated at 4℃ overnight at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KMT6 /EZH2 antibody [11/EZH2] (ab283270) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

KMT6 /EZH2 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG H&L (IRDye® 800CW) (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-KMT6/EZH2 antibody [11/EZH2] - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

11/EZH2

亚型

IgG1

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column

产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KMT6 also known as EZH2 (Enhancer of Zeste Homolog 2) is a protein that functions as a histone methyltransferase. It catalyzes the methylation of histone H3 on lysine 27 (H3K27) a modification associated with gene silencing. EZH2 is a component of the Polycomb Repressive Complex 2 (PRC2) which plays an important role in chromatin remodeling and transcriptional repression. The EZH2 protein has a molecular weight of approximately 87 kDa and its gene is located on chromosome 7. In terms of expression EZH2 is found in various tissues but is notably abundant in growing and dividing cells.
Biological function summary

EZH2 plays a central role in regulating gene expression during development and differentiation. It is a core component of the PRC2 complex which includes other proteins such as EED and SUZ12. Through its catalytic activity EZH2 contributes to the maintenance of the transcriptionally repressed state of genes influencing processes such as cell identity and proliferation. In many cell types EZH2-mediated gene silencing is necessary for normal development and dysregulation can lead to aberrant cellular processes.

Pathways

EZH2 participates in key regulatory networks notably the Wnt signaling pathway and the p53 pathway. Within the Wnt pathway EZH2's activity contributes to controlling gene expression that influences cellular differentiation and proliferation. Meanwhile interaction with the p53 pathway can impact the cellular response to stress and DNA damage highlighting EZH2’s regulatory dimension. Other proteins like β-catenin in the Wnt pathway interact with EZH2 reflecting its integration in these complex signaling networks.

EZH2 has been linked to cancer and other proliferative diseases. Overexpression or mutation of EZH2 is frequently observed in cancers such as prostate and breast cancer where it contributes to unchecked cell growth and tumor progression. In these contexts proteins like p53 can interact abnormally with EZH2 leading to failure in tumor suppression mechanisms. Additionally deregulated EZH2 activity is implicated in certain developmental disorders highlighting its broader impact on cellular and organismal homeostasis.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 14532106, PubMed : 15225548, PubMed : 15385962, PubMed : 16618801, PubMed : 16936726, PubMed : 17344414, PubMed : 22323599, PubMed : 24474760, PubMed : 26581166, PubMed : 30026490, PubMed : 30923826). Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively (PubMed : 15231737, PubMed : 17210787, PubMed : 18285464, PubMed : 22323599, PubMed : 30923826). Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed : 22323599, PubMed : 30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation (PubMed : 19026781). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 16357870, PubMed : 17200670). Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes (PubMed : 16179254, PubMed : 18086877, PubMed : 20935635). EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA (PubMed : 23063525). Regulates the circadian clock via histone methylation at the promoter of the circadian genes (PubMed : 16717091). Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription (By similarity).
See full target information EZH2
style
left-column

文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1040 PubMed41034982

2025

Integrated single-cell RNA-seq analysis reveals that EZH2 regulates the MIF-CD74 axis to modulate T cell activation and exhaustion in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Zhou,Yi Xu,Mengfan Ye,Jiayi Xing,Di Chen,Al-Ameer Wail Hussein Ahmed,Al-Rahabi Rehab Abdullah Mohammed Ali,Chenwei Pan,Xiangchou Yang,Zan Shen

Investigative ophthalmology & visual science 66:59 PubMed40856653

2025

Single-Cell Transcriptomics Reveals Effects of Long-Term Contact Lens Wearing on the Human Corneal Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Zhang,Di Sun,Ting Liu,Ning Wang,Shengqian Dou,Qun Wang,Dewei Li,Yi Song,Le Tian,Luqin Wan,Min Chen

Cancer biology & therapy 26:2529652 PubMed40619273

2025

USP44 promotes chemotherapeutic drug resistance of triple negative breast cancer through EZH2 protein stability.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Wu,Wanting Xiao,Junjie Ni,Yuming Lou,Chaoyang Xu

Endocrine 84:1146-1153 PubMed38340242

2024

The significance of an immunohistochemical marker-based panel in assisting the diagnosis of parathyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Hu,Shengwei Mo,Jinheng Xiao,Ming Cui,Qingyuan Zheng,Tianqi Chen,Xiaoyan Chang,Quan Liao

PloS one 18:e0294729 PubMed37988356

2023

Long non-coding RNA SNHG17 may function as a competitive endogenous RNA in diffuse large B-cell lymphoma progression by sponging miR-34a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shengjuan Lu,Lin Zeng,Guojun Mo,Danqing Lei,Yuanhong Li,Guodi Ou,Hailian Wu,Jie Sun,Chao Rong,Sha He,Dani Zhong,Qing Ke,Qingmei Zhang,Xiaohong Tan,Hong Cen,Xiaoxun Xie,Chengcheng Liao

Cells 12: PubMed37887281

2023

Enhanced Transcriptional Signature and Expression of Histone-Modifying Enzymes in Salivary Gland Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Manou,Theodoros Loupis,Dimitrios M Vrachnos,Nikolaos Katsoulas,Stamatios Theocharis,Dimitrios S Kanakoglou,Efthimia K Basdra,Christina Piperi,Athanasios G Papavassiliou

Environmental toxicology 38:2240-2255 PubMed37334851

2023

Histone methyltransferase enzyme enhancer of zeste homolog 2 counteracts ischemic brain injury via H3K27me3-mediated regulation of PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Chen,Limin Fan,Guoping Wu,Hairong Wang,Shuo Gu

International journal of rheumatic diseases 26:638-647 PubMed36789537

2023

Long non-coding RNA telomerase RNA elements improve glucocorticoid-induced osteoporosis by EZH2 to regulate DKK1.

Applications

Unspecified application

Species

Unspecified reactive species

He Hu,Xiaodong Guo,Tingting Mu,Huifang Song

Signal transduction and targeted therapy 8:18 PubMed36642705

2023

Discovery of IHMT-337 as a potent irreversible EZH2 inhibitor targeting CDK4 transcription for malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Husheng Mei,Hong Wu,Jing Yang,Bin Zhou,Aoli Wang,Chen Hu,Shuang Qi,Zongru Jiang,Fengming Zou,Beilei Wang,Feiyang Liu,Yongfei Chen,Wenchao Wang,Jing Liu,Qingsong Liu

International journal of medical sciences 20:114-124 PubMed36619221

2023

EZH2 promotes angiogenesis in peritoneal dialysis by epigenetically activating SP4 expression in the IL-6/sIL-6R signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhu,Haochen Guan,Xuan Wang,Yueyue Zhang,Lijie Gu,Jieshuang Jia,Ling Wang,Weijie Yuan
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB6002

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

  • 4
  • 72
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com