Anti-KMT4 / Dot1L抗体(ab72454)
Key features and details
- Rabbit polyclonal to KMT4 / Dot1L
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KMT4 / Dot1L抗体
参阅全部 KMT4 / Dot1L 一抗 -
描述
兔多克隆抗体to KMT4 / Dot1L -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human KMT4/ Dot1L aa 1000-1100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8TEK3 -
阳性对照
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. -
序列相似性
Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Contains 1 DOT1 domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 84444 Human
- Omim: 607375 Human
- SwissProt: Q8TEK3 Human
- Unigene: 713641 Human
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别名
- Disrupter of telomere silencing protein 1 antibody
- DOT 1 antibody
- DOT1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KMT4 / Dot1L with ab72454 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-KMT4 / Dot1L antibody (ab72454) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 185 kDa
Observed band size: 185 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
HeLa whole cell lysate ( 1 mg for IP, 20% of IP loaded) with ab72454 at 3 µg/mg lysate for IP, followed by ab72454 at 1 µg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab72454 被引用在 15 文献中.
- Si Y et al. A novel screening strategy to identify histone methyltransferase inhibitors reveals a crosstalk between DOT1L and CARM1. RSC Chem Biol 3:456-467 (2022). PubMed: 35441144
- Salvati A et al. Combinatorial targeting of a chromatin complex comprising Dot1L, menin and the tyrosine kinase BAZ1B reveals a new therapeutic vulnerability of endocrine therapy-resistant breast cancer. Breast Cancer Res 24:52 (2022). PubMed: 35850772
- Alexandrova E et al. Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer. Cancer Cell Int 22:336 (2022). PubMed: 36333801
- Vatapalli R et al. Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer. Nat Commun 11:4153 (2020). PubMed: 32814769
- Vilema-Enríquez G et al. Inhibition of the SUV4-20 H1 histone methyltransferase increases frataxin expression in Friedreich's ataxia patient cells. J Biol Chem 295:17973-17985 (2020). PubMed: 33028632