Anti-KMT3C / SMYD2抗体(ab108217)
Key features and details
- Rabbit polyclonal to KMT3C / SMYD2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KMT3C / SMYD2抗体
参阅全部 KMT3C / SMYD2 一抗 -
描述
兔多克隆抗体to KMT3C / SMYD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, 293T and NIH 3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab108217 was affinity purified using an epitope specific to KMT3C / SMYD2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. -
序列相似性
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
细胞定位
Cytoplasm > cytosol. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 421361 Chicken
- Entrez Gene: 615229 Cow
- Entrez Gene: 56950 Human
- Entrez Gene: 226830 Mouse
- Entrez Gene: 100294706 Pig
- Omim: 610663 Human
- SwissProt: E1C5V0 Chicken
- SwissProt: Q0P585 Cow
see all -
别名
- Histone methyltransferase SMYD2 antibody
- HSKM B antibody
- HSKM-B antibody
see all
图片
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Western blot - Anti-KMT3C / SMYD2 antibody (ab108217)All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 0.1 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes -
Western blot - Anti-KMT3C / SMYD2 antibody (ab108217)This image is courtesy of an anonymous AbreviewAll lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 1/1000 dilution
Lane 1 : U2OS whole cell lysate - siRNA targeting luciferase
Lanes 2-3 : U2OS whole cell lysate - siRNA targeting SMYD2
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 20 secondsBlocked with 5% BSA for 1 hour at room temperature.
Primary antibody incubation time: 1 hour.
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Immunoprecipitation - Anti-KMT3C / SMYD2 antibody (ab108217)Detection of KMT3C / SMYD2 by Western blot of Immunprecipitate.
ab108217, at 1 µg/ml, staining KMT3C / SMYD2 in HeLa whole cell lysate immunoprecipitated using ab108217 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108217 被引用在 7 文献中.
- Szulik MW et al. SMYD1a protects the heart from ischemic injury by regulating OPA1-mediated cristae remodeling and supercomplex formation. Basic Res Cardiol 118:20 (2023). PubMed: 37212935
- Zhou Y et al. SMYD2 regulates vascular smooth muscle cell phenotypic switching and intimal hyperplasia via interaction with myocardin. Cell Mol Life Sci 80:264 (2023). PubMed: 37615725
- Yu YQ et al. SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth. Cell Death Dis 13:52 (2022). PubMed: 35022391
- Sun JJ et al. SMYD2 promotes cervical cancer growth by stimulating cell proliferation. Cell Biosci 9:75 (2019). PubMed: 31548876
- Eggert E et al. Discovery and Characterization of a Highly Potent and Selective Aminopyrazoline-Based in Vivo Probe (BAY-598) for the Protein Lysine Methyltransferase SMYD2. J Med Chem 59:4578-600 (2016). PubMed: 27075367
- Xu G et al. The histone methyltransferase Smyd2 is a negative regulator of macrophage activation by suppressing interleukin 6 (IL-6) and tumor necrosis factor a (TNF-a) production. J Biol Chem 290:5414-23 (2015). ChIP ; Mouse . PubMed: 25583990
- Liu T et al. Master redox regulator Trx1 upregulates SMYD1 & modulates lysine methylation. Biochim Biophys Acta 1854:1816-22 (2015). WB ; Human . PubMed: 26410624