重组Anti-Klotho抗体[EPR6856] (ab181373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6856] to Klotho
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Klotho抗体[EPR6856]
参阅全部 Klotho 一抗 -
描述
兔单克隆抗体[EPR6856] to Klotho -
宿主
Rabbit -
特异性
When we tested the antibody in human samples, the only form of the protein that we are able to detect is the secreted one (about 65kDa). When we tested the antibody in mouse samples (wt vs KO) we could only detect the membrane bound form of the protein (about 130kDa). This difference could be due to the nature of the samples (different species). However, we do not have a clear explanation about this. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented here. -
经测试应用
适用于: IHC-P, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Klotho aa 400-500. The exact sequence is proprietary.
Database link: Q9UEF7 -
阳性对照
- Human testis, fetal kidney and fetal thymus lysates; Human clear cell carcinoma kidney tissue, Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6856 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181373于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified, use 1/100 - 1/250. |
WB |
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62, 116 kDa).
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说明 |
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IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified, use 1/100 - 1/250. |
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62, 116 kDa). |
靶标
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功能
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. -
组织特异性
Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney. -
疾病相关
Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. -
序列相似性
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. -
结构域
Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide. - Information by UniProt
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数据库链接
- Entrez Gene: 9365 Human
- Entrez Gene: 16591 Mouse
- Omim: 604824 Human
- SwissProt: Q9UEF7 Human
- SwissProt: O35082 Mouse
- Unigene: 524953 Human
- Unigene: 6500 Mouse
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别名
- Kl antibody
- KLOT_HUMAN antibody
- Klotho peptide antibody
图片
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 5 µg/ml
Lane 1 : Wild Type (WT) Mouse Kidney Tissue Lysate
Lane 2 : Knock Out (KO) Mouse Kidney Tissue Lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 62, 116 kDaThe blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373 (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1:10,000 dilutions for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (purified)
Lane 1 : Human fetal kidney
Lane 2 : Human testis
Lane 3 : Human fetal thymus
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 62, 116 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney lysates
Lane 2 : Human testis lysates
Lane 3 : Human fetal thymus lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 62, 116 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (38)
ab181373 被引用在 38 文献中.
- Wang Q et al. Inhibition of EZH2 mitigates peritoneal fibrosis and lipid precipitation in peritoneal mesothelial cells mediated by klotho. Ren Fail 45:2149411 (2023). PubMed: 36724065
- Ren HL et al. Growth hormone-releasing hormone agonist attenuates vascular calcification in diabetic db/db mice. Front Cardiovasc Med 10:1102525 (2023). PubMed: 36742073
- Donate-Correa J et al. Klotho inversely relates with carotid intima- media thickness in atherosclerotic patients with normal renal function (eGFR ≥60 mL/min/1.73m2): a proof-of-concept study. Front Endocrinol (Lausanne) 14:1146012 (2023). PubMed: 37274332
- Kunke M et al. Targeted deletion of von-Hippel-Lindau in the proximal tubule conditions the kidney against early diabetic kidney disease. Cell Death Dis 14:562 (2023). PubMed: 37626062
- Pan KH et al. KL is a favorable prognostic factor related immune for clear cell renal cell carcinoma. Eur J Med Res 28:356 (2023). PubMed: 37726833