AB314431

重组Anti-KLF2抗体[EPR23420-40] - BSA and Azide free

Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal KLF2 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

查看别名

Lklf, Klf2, Krueppel-like factor 2, Lung krueppel-like factor

3 Images

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed using ab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the bands above 38 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1 : 37 seconds Lane 2 : 59 seconds

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (<a href='/products/primary-antibodies/klf2-antibody-epr23420-40-ab314430'>ab314430</a>) at 1/1000 dilution

Lane 1:

ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed usingab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (<a href='/products/primary-antibodies/klf2-antibody-epr23420-40-ab314430'>ab314430</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 µg at 20 µg

Lane 2:

F9 transfected with siRNAs specifically targeting KLF2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed usingab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : brain, retina, liver. The identity of the bands above 38 kDa and around 18 kDa are unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (<a href='/products/primary-antibodies/klf2-antibody-epr23420-40-ab314430'>ab314430</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse retina tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (<a href='/products/primary-antibodies/gapdh-antibody-epr16891-loading-control-ab181602'>ab181602</a>) at 1/200000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 81s

不同偶联物与剂型 (1)

Unconjugated

Anti-KLF2 antibody [EPR23420-40]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23420-40

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KLF2 also known as Kruppel-like factor 2 is a transcription factor with a molecular mass of approximately 37 kDa. It belongs to the Kruppel-like family of transcription factors which share zinc finger domains. KLF2 regulates gene expression by binding to specific DNA sequences in promoters influencing cellular processes. Researchers have localized its expression predominantly in the vascular endothelium the lungs and the spleen. In these tissues KLF2 influences endothelial cell function and vascular biology.

Biological function summary

KLF2 mediates cellular processes including endothelial cell maintenance and vascular development. As a transcription factor KLF2 does not operate as part of a multi-protein complex but rather performs its regulatory function individually by modulating target gene expression. KLF2 activates genes associated with anti-inflammatory and anti-thrombotic properties in endothelial cells contributing to vascular homeostasis. Its role is key in maintaining the balance between pro- and anti-inflammatory signals in the vascular system.

Pathways

KLF2 integrates its function into various signaling pathways notably the MEK/ERK and PI3K/Akt pathways. KLF2 modulates these pathways to control proliferation differentiation and apoptosis in endothelial cells. In particular KLF2-induced gene expression influences these pathways by interacting with other proteins such as MEK and ERK proteins that regulate cell survival and inflammatory responses. These interactions highlight the relevance of KLF2 in cellular signaling important for vascular health.

KLF2 has significant implications for cardiovascular diseases and atherosclerosis. Research associates KLF2 with protective roles against the development of atherosclerosis due to its influence on endothelial function and inflammation. When KLF2 expression is compromised the risk for thrombosis and vascular inflammation increases contributing to cardiovascular conditions. Additionally studies link KLF2 to the pathophysiology of diabetes where aberrant KLF2 expression disrupts normal endothelial functions exacerbating diabetic complications. KLF2 interacts with proteins like NF-kB in such conditions impacting the expression of genes involved in inflammation and metabolism.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements (By similarity).

See full target information Klf2

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-KLF2抗体[EPR23420-40] - BSA and Azide free