Anti-KLF15抗体(ab2647)
Key features and details
- Goat polyclonal to KLF15
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KLF15抗体
参阅全部 KLF15 一抗 -
描述
山羊多克隆抗体to KLF15 -
宿主
Goat -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig
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免疫原
Synthetic peptide within Human KLF15 aa 1-100 (N terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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常规说明
Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)
We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration. PubMed: 24407292
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| WB |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31). |
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| IHC-P |
Use at an assay dependent concentration. PubMed: 26378916
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| 说明 |
|---|
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IP
Use at an assay dependent concentration. PubMed: 24407292 |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa). A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31). |
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IHC-P
Use at an assay dependent concentration. PubMed: 26378916 |
靶标
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功能
Transcriptional activator. Binds to the GA element of the CLCNKA promoter. -
组织特异性
Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues. -
序列相似性
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 28999 Human
- Entrez Gene: 66277 Mouse
- Entrez Gene: 85497 Rat
- GenBank: NP_054798.1 Human
- Omim: 606465 Human
- SwissProt: Q9UIH9 Human
- SwissProt: Q9EPW2 Mouse
- Unigene: 272215 Human
see all -
别名
- Kidney enriched Kruppel like factor antibody
- Kidney-enriched krueppel-like factor antibody
- KKLF antibody
see all
图片
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Western blot - Anti-KLF15 antibody (ab2647)Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml
Lane 1 : HepG2 nuclear lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 44 kDa
数据表及文件
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Datasheet download
文献 (23)
ab2647 被引用在 23 文献中.
- Wang ZH et al. Circular RNA circFBXO7 attenuates non-small cell lung cancer tumorigenesis by sponging miR-296-3p to facilitate KLF15-mediated transcriptional activation of CDKN1A. Transl Oncol 30:101635 (2023). PubMed: 36774884
- Gao F et al. A defect in mitochondrial protein translation influences mitonuclear communication in the heart. Nat Commun 14:1595 (2023). PubMed: 36949106
- Yoo JY et al. Loss of MIG-6 results in endometrial progesterone resistance via ERBB2. Nat Commun 13:1101 (2022). PubMed: 35232969
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Ostler JB et al. Transactivation of Herpes Simplex Virus 1 (HSV-1) Infected Cell Protein 4 Enhancer by Glucocorticoid Receptor and Stress-Induced Transcription Factors Requires Overlapping Krüppel-Like Transcription Factor 4/Sp1 Binding Sites. J Virol 95:N/A (2021). PubMed: 33208447
