AB2647

Anti-KLF15抗体

Name: KLF15抗体 (ab2647)| Abcam中文官网
Brand: Abcam Limited
SKU: AB2647
Price: 5050 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-KLF15 antibody

(1 Review)

(29 Publications)

Goat Polyclonal KLF15 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human KLF15 aa 1-50.

查看别名

KKLF, KLF15, Krueppel-like factor 15, Kidney-enriched krueppel-like factor

1 Images

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Western blot - Anti-KLF15 antibody (AB2647)

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Western blot - Anti-KLF15 antibody (ab2647) at 1 µg/mL

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Western blot - Anti-KLF15 antibody (ab2647) at 0.5 µg/mL

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HepG2 nuclear lysate at 35 µg

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HeLa cell lysate at 35 µg

Predicted band size: 44 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P, IP

applications

免疫原

Synthetic Peptide within Human KLF15 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UIH9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KLF15 also known as Kruppel-like factor 15 works mechanically as a transcription factor. The KLF15 protein with a molecular mass of approximately 44 kDa functions by regulating the expression of genes involved in metabolism and cell differentiation. It is expressed in various tissues including the heart liver and skeletal muscle. This expression suggests that the KLF-15 protein plays a significant role in maintaining tissue-specific functions.

Biological function summary

KLF15 influences several cellular processes by regulating gene expression. It is part of a transcription factor complex that modulates glucose metabolism lipid metabolism and muscle differentiation. The KLF-15 protein contributes to maintaining homeostasis by upregulating genes responsible for gluconeogenesis and lipid usage which is essential in adapting to fasting states. Additionally it impacts muscle cell differentiation by influencing the expression of specific myogenic factors.

Pathways

KLF15 is significant in metabolic and myogenic pathways. It upregulates genes involved in the gluconeogenesis pathway and fatty acid oxidation collaborating with proteins such as PGC-1α. Additionally within myogenic pathways KLF-15 protein interacts with myogenic regulatory factors contributing to muscle differentiation. These interactions highlight its importance in metabolic and muscle function pathways and highlight its regulatory capacity across different biological processes.

KLF15 has important roles. Alterations in KLF-15 protein levels link to metabolic diseases such as diabetes and cardiac hypertrophy. In diabetes it affects glucose metabolism and insulin sensitivity with connections to proteins like FOXO1. In cardiac hypertrophy KLF-15 influences structural remodeling of the heart potentially interacting with hypertrophy-associated factors. This connection to diseases emphasizes its potential as a therapeutic target for related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.

See full target information KLF15

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:270 PubMed40490444

2025

Inhibition of epigenetic regulator UHRF1 attenuates renal fibrosis and retains transcription factor Krüppel-like factor 15 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yulu Gu,Shiqi Lv,Xinhui Huang,Jialin Wang,Yulin Wang,Han Zhang,Ziyan Shen,Jing Chen,Cheng Zhu,Di Zhang,Xiaoqiang Ding,Xiaoyan Zhang

Genes & diseases 12:101545 PubMed40485981

2025

Enhancer profiling uncovers Jmjd1c as an essential suppressor in neuropathic pain by targeting Socs3.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Yan Xie,Shun Wang,Moxuan Gong,Zheping Chen,Chuanxin Wang,Peilong Li

Molecular brain 18:3 PubMed39838470

2025

Distribution and functional significance of KLF15 in mouse cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Shuijing Cao,Yanrong Chen,Yueyan Liu,Kugeng Huo,Zhuangqi Shi,Shuxin Han,Liecheng Wang

The Journal of clinical investigation 134: PubMed38949025

2024

White adipocytes in subcutaneous fat depots require KLF15 for maintenance in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Li,Brian J Feldman

Communications biology 7:130 PubMed38273088

2024

KLF15 transcriptionally activates LINC00689 to inhibit colorectal cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Cao,Jian Li,Gang Zhang,Hao Fang,Yongliang Du,Yan Liang

Nature communications 14:1595 PubMed36949106

2023

A defect in mitochondrial protein translation influences mitonuclear communication in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Tian Liang,Yao Wei Lu,Xuyang Fu,Xiaoxuan Dong,Linbin Pu,Tingting Hong,Yuxia Zhou,Yu Zhang,Ning Liu,Feng Zhang,Jianming Liu,Andrea P Malizia,Hong Yu,Wei Zhu,Douglas B Cowan,Hong Chen,Xinyang Hu,John D Mably,Jian'an Wang,Da-Zhi Wang,Jinghai Chen

Translational oncology 30:101635 PubMed36774884

2023

Circular RNA circFBXO7 attenuates non-small cell lung cancer tumorigenesis by sponging miR-296-3p to facilitate KLF15-mediated transcriptional activation of CDKN1A.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Hao Wang,Lin-Lin Ye,Xuan Xiang,Xiao-Shan Wei,Yi-Ran Niu,Wen-Bei Peng,Si-Yu Zhang,Pei Zhang,Qian-Qian Xue,Hao-Lei Wang,Yi-Heng Du,Yao Liu,Jia-Qi Ai,Qiong Zhou

Molecular medicine reports 26: PubMed35656890

2022

CTRP12 alleviates cardiomyocyte ischemia‑reperfusion injury via regulation of KLF15.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liao,Xiaoyuan Tian

iScience 25:104184 PubMed35494220

2022

Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Leroy C Joseph,Jianting Shi,Quynh N Nguyen,Victoria Pensiero,Chris Goulbourne,Robert C Bauer,Hanrui Zhang,John P Morrow

Nature communications 13:1101 PubMed35232969

2022

Loss of MIG-6 results in endometrial progesterone resistance via ERBB2.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Yoon Yoo,Tae Hoon Kim,Jung-Ho Shin,Ryan M Marquardt,Ulrich Müller,Asgerally T Fazleabas,Steven L Young,Bruce A Lessey,Ho-Geun Yoon,Jae-Wook Jeong

View all publications

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Anti-KLF15抗体

Anti-KLF15 antibody

Western blot - Anti-KLF15 antibody (AB2647)