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重组RabMAb

重组Anti-KLC3抗体[EPR11149(2)] (ab180523)

Submit a review Submit a question References (4)
Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
  • Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
  • Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
  • Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
  • Anti-KLC3 antibody [EPR11149(2)] (ab180523)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11149(2)] to KLC3
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-KLC3抗体[EPR11149(2)]
    参阅全部 KLC3 一抗

  • 描述

    兔单克隆抗体[EPR11149(2)] to KLC3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human KLC3 aa 350-450. The exact sequence is proprietary.
    Database link: Q6P597

  • 阳性对照

    • Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR11149(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180523于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
说明
ICC/IF
1/1000.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.

靶标

  • 功能

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.

  • 序列相似性

    Belongs to the kinesin light chain family.
    Contains 5 TPR repeats.

  • 结构域

    The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding.

  • 细胞定位

    Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Kinesin light chain 2 antibody
      • Kinesin light chain 3 antibody
      • KLC2 antibody
      • KLC2 like antibody
      • KLC2-like antibody
      • KLC2L antibody
      • KLC3 antibody
      • KLC3_HUMAN antibody
      • KLCt antibody
      • KNS2B antibody
      see all

    图片

    • Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : U-87 MG cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use)  HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

      Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.

    • Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

      Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.

    • Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

      Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed)labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC)at 1/75 dilution was used as the secondary antibody.Rabbit monoclonal IgG was used as the isotype control. 

       

       

    • Anti-KLC3 antibody [EPR11149(2)] (ab180523)
      Anti-KLC3 antibody [EPR11149(2)] (ab180523)

    文献 (4)

    发表研究结果有使用 ab180523？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab180523 被引用在 4 文献中.

    • Rah G  et al. KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease. J Am Soc Nephrol 33:1726-1741 (2022). PubMed: 35961787
    • Chung J  et al. Super-resolution imaging of platelet-activation process and its quantitative analysis. Sci Rep 11:10511 (2021). PubMed: 34006947
    • Go S  et al. Super-resolution imaging reveals cytoskeleton-dependent organelle rearrangement within platelets at intermediate stages of maturation. Structure 29:810-822.e3 (2021). PubMed: 34143977
    • Georgiades P  et al. The flexibility and dynamics of the tubules in the endoplasmic reticulum. Sci Rep 7:16474 (2017). PubMed: 29184084

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