Anti-Kir6.2/BIR抗体(ab79171)
Key features and details
- Rabbit polyclonal to Kir6.2/BIR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Kir6.2/BIR抗体
参阅全部 Kir6.2/BIR 一抗 -
描述
兔多克隆抗体to Kir6.2/BIR -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, PBS, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
靶标
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相关性
ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus. -
细胞定位
Cell Membrane; Multi-pass membrane protein -
数据库链接
- Entrez Gene: 3767 Human
- Entrez Gene: 16514 Mouse
- Omim: 600937 Human
- SwissProt: Q14654 Human
- SwissProt: Q61743 Mouse
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别名
- ATP sensitive inward rectifier potassium channel 11 antibody
- Beta cell inward rectifier subunit antibody
- BIR antibody
see all
图片
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Western blot - Anti-Kir6.2/BIR antibody (ab79171)All lanes : Anti-Kir6.2/BIR antibody (ab79171) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated with heat shock
Lane 2 : extracts from NIH3T3 cells treated with heat shock
Lane 3 : extracts from NIH3T3 cells treated with heat shock with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody (ab79171)Immunohistochemical analysis of Kir6.2/BIR in paraffin embedded human brain tissue using a 1/50 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Kir6.2/BIR antibody (ab79171)Immunofluorescence analysis of Kir6.2/BIR in HeLa cells, using a 1/500 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab79171 被引用在 8 文献中.
- Wei C et al. The reversible effects of free fatty acids on sulfonylurea-stimulated insulin secretion are related to the expression and dynamin-mediated endocytosis of KATP channels in pancreatic β cells. Endocr Connect 12:N/A (2023). PubMed: 36398885
- Maqoud F et al. Immunohistochemical, pharmacovigilance, and omics analyses reveal the involvement of ATP-sensitive K+ channel subunits in cancers: role in drug-disease interactions. Front Pharmacol 14:1115543 (2023). PubMed: 37180726
- Jiang Z et al. Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes. EMBO J 41:e106825 (2022). PubMed: 35023164
- Bai XJ et al. Glucagon-Like Peptide-1 Analog Liraglutide Attenuates Pressure-Overload Induced Cardiac Hypertrophy and Apoptosis through Activating ATP Sensitive Potassium Channels. Cardiovasc Drugs Ther 35:87-101 (2021). PubMed: 33057968
- Okada T et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
- Hu D et al. Identification, clinical manifestation and structural mechanisms of mutations in AMPK associated cardiac glycogen storage disease. EBioMedicine 54:102723 (2020). PubMed: 32259713
- Gong XG et al. Da-bu-yin-wan and qian-zheng-san to neuroprotect the mouse model of Parkinson's disease. Evid Based Complement Alternat Med 2014:729195 (2014). WB . PubMed: 25610480
- Raeis-Dauvé V et al. Increased expression of adenosine triphosphate-sensitive K+ channels in mitral dysfunction: mechanically stimulated transcription and hypoxia-induced protein stability? J Am Coll Cardiol 59:390-6 (2012). PubMed: 22133355