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重组RabMAb

重组Anti-Kininogen 1抗体[EPR6097] - BSA and Azide free (ab226087)

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Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6097] to Kininogen 1 - BSA and Azide free
  • Suitable for: ELISA, WB, IP, IHC-P
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Kininogen 1抗体[EPR6097] - BSA and Azide free
    参阅全部 Kininogen 1 一抗

  • 描述

    兔单克隆抗体[EPR6097] to Kininogen 1 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This antibody detects three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).    

  • 经测试应用

    适用于: ELISA, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human, Recombinant fragment

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human plasma, platelet and ovarian cancer lysates; Human kidney tissue, Human ovarian adenocarcinoma tissue.

  • 常规说明

    ab226087 is the carrier-free version of ab124737.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 解离常数（KD）

    KD = 2.70 x 10 -12 M
    Learn more about KD

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR6097

  • 同种型

    IgG

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226087于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 45, 56, 120 kDa (predicted molecular weight: 48, 72 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 45, 56, 120 kDa (predicted molecular weight: 48, 72 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

  • 组织特异性

    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

  • 疾病相关

    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

  • 序列相似性

    Contains 3 cystatin domains.

  • 翻译后修饰

    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • 细胞定位

    Secreted > extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Alpha-2-thiol proteinase inhibitor antibody
      • BDK antibody
      • BK antibody
      • Bradykinin antibody
      • Bradykinin included antibody
      • Fitzgerald factor antibody
      • FLAUJEAC FACTOR antibody
      • High molecular weight kininogen antibody
      • HMWK antibody
      • Ile-Ser-Bradykinin antibody
      • Kallidin I antibody
      • Kallidin II antibody
      • KNG antibody
      • KNG1 antibody
      • KNG1_HUMAN antibody
      • Low molecular weight growth-promoting factor antibody
      • WILLIAMS FACTOR antibody
      • Williams-Fitzgerald-Flaujeac factor antibody
      see all

    图片

    • Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      All lanes : Anti-Kininogen 1 antibody [EPR6097] (ab124737) at 1/10000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human platelet tissue lysate
      Lane 3 : Human ovarian cancer tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 48, 72 kDa



      This data was developed using ab124737, the same antibody clone in a different buffer formulation.

      ab124737 is detecting three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human kidney tissues by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

      This data was developed using ab124737, the same antibody clone in a different buffer formulation.

      ELISA analysis of Human Kininogen-1 recombinant protein at 1000 ng/mL with ab124737. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226087？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226087 尚未被引用在任何文献中。

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