Anti-Kindlin 2抗体(ab74030)
Key features and details
- Rabbit polyclonal to Kindlin 2
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Kindlin 2抗体
参阅全部 Kindlin 2 一抗 -
描述
兔多克隆抗体to Kindlin 2 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human Kindlin 2 aa 450-550 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab74029) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
|
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
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| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IP
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. -
组织特异性
Ubiquitous. Found in numerous tumor tissues. -
序列相似性
Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain. -
结构域
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. -
细胞定位
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites. - Information by UniProt
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数据库链接
- Entrez Gene: 616981 Cow
- Entrez Gene: 10979 Human
- Entrez Gene: 218952 Mouse
- Entrez Gene: 289992 Rat
- Omim: 607746 Human
- SwissProt: Q96AC1 Human
- SwissProt: Q8CIB5 Mouse
- Unigene: 509343 Human
see all -
别名
- FLJ34213 antibody
- FLJ44462 antibody
- UNC112 antibody
see all
图片
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Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FERMT2 knockout HeLa cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-3: Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265247 (knockout cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8 minutes -
Immunoprecipitation - Anti-Kindlin 2 antibody (ab74030)Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 78kDa; Kindlin 2 -
Western blot - Anti-Kindlin 2 antibody (ab74030)This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United StatesThe kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab74030 被引用在 14 文献中.
- Xia W et al. Alzheimer's risk factor FERMT2 promotes the progression of colorectal carcinoma via Wnt/β-catenin signaling pathway and contributes to the negative correlation between Alzheimer and cancer. PLoS One 17:e0278774 (2022). PubMed: 36480537
- Cui Q et al. YBX1 knockdown induces renal cell carcinoma cell apoptosis via Kindlin-2. Cell Cycle 20:2413-2427 (2021). PubMed: 34709966
- Boscher E et al. MicroRNA-138 Overexpression Alters Aß42 Levels and Behavior in Wildtype Mice. Front Neurosci 14:591138 (2020). PubMed: 33519353
- Boscher E et al. Copy Number Variants in miR-138 as a Potential Risk Factor for Early-Onset Alzheimer's Disease. J Alzheimers Dis 68:1243-1255 (2019). PubMed: 30909216
- Wei CY et al. Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma. Aging (Albany NY) 11:6273-6285 (2019). PubMed: 31427543