重组Anti-KIFAP3抗体[EPR5600] (ab133537)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5600] to KIFAP3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KIFAP3抗体[EPR5600]
参阅全部 KIFAP3 一抗 -
描述
兔单克隆抗体[EPR5600] to KIFAP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human KIFAP3 aa 700-800. The exact sequence is proprietary.
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阳性对照
- HeLa, SH-SY5Y, C6, and 293T cell lysates; and Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.05 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5600 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex. -
序列相似性
Contains 5 ARM repeats. -
翻译后修饰
Phosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1. - Information by UniProt
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数据库链接
- Entrez Gene: 22920 Human
- Entrez Gene: 16579 Mouse
- Entrez Gene: 289168 Rat
- Omim: 601836 Human
- SwissProt: Q92845 Human
- SwissProt: P70188 Mouse
- SwissProt: D3ZK15 Rat
- SwissProt: D3ZWA5 Rat
see all -
别名
- FLJ22818 antibody
- KAP 3 antibody
- KAP-3 antibody
see all
图片
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All lanes : Anti-KIFAP3 antibody [EPR5600] (ab133537) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 91 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] (ab133537)
Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133537 被引用在 1 文献中.
- Li S et al. Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control in Chlamydomonas. Elife 9:N/A (2020). PubMed: 33112235