Anti-KIF5C抗体(ab5630)
Key features and details
- Rabbit polyclonal to KIF5C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KIF5C抗体
参阅全部 KIF5C 一抗 -
描述
兔多克隆抗体to KIF5C -
宿主
Rabbit -
特异性
This antibody is specific for KIF5C and does not detect other kinesin isotypes.
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human KIF5C aa 938-957.
Sequence:AVHAIRGGGGSSSNSTHYQK
(Peptide available asab41784) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (4) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
靶标
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功能
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
组织特异性
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary. -
序列相似性
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
结构域
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 3800 Human
- Omim: 604593 Human
- SwissProt: O60282 Human
- Unigene: 435557 Human
- Unigene: 660699 Human
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别名
- CDCBM2 antibody
- FLJ44735 antibody
- KIAA0531 antibody
see all
图片
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Shows a Western blot of KIF5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.
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IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab5630 被引用在 16 文献中.
- Brachet A et al. A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression. Cell Rep 36:109499 (2021). PubMed: 34348158
- Zhang W et al. Formin Activity and mDia1 Contribute to Maintain Axon Initial Segment Composition and Structure. Mol Neurobiol 58:6153-6169 (2021). PubMed: 34458961
- Swarnkar S et al. Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation. Cell Rep 36:109369 (2021). PubMed: 34260917
- Pegg CE et al. Herpesviruses assimilate kinesin to produce motorized viral particles. Nature 599:662-666 (2021). PubMed: 34789877
- Furey C et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393