为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin

Anti-KIF5C抗体(ab5630)

  • Datasheet
Reviews (5)Q&A (1)References (14)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-KIF5C antibody (ab5630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)

Key features and details

  • Rabbit polyclonal to KIF5C
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-KIF5C antibody [EPR16224-32] (ab193352)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-KIF5C抗体
    参阅全部 KIF5C 一抗
  • 描述

    兔多克隆抗体to KIF5C
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific for KIF5C and does not detect other kinesin isotypes.

  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human KIF5C aa 938-957.
    Sequence:

    AVHAIRGGGGSSSNSTHYQK


    (Peptide available as ab41784)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5630于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (4)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

靶标

  • 功能

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • 组织特异性

    Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
  • 序列相似性

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • 结构域

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession O60282 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3800 Human
    • Omim: 604593 Human
    • SwissProt: O60282 Human
    • Unigene: 435557 Human
    • Unigene: 660699 Human
    • 别名

      • CDCBM2 antibody
      • FLJ44735 antibody
      • KIAA0531 antibody
      • KIF 5C antibody
      • Kif5c antibody
      • KIF5C protein antibody
      • KIF5C_HUMAN antibody
      • Kinesin family member 5C antibody
      • Kinesin heavy chain isoform 5C antibody
      • Kinesin heavy chain neuron specific 2 antibody
      • Kinesin heavy chain neuron specific antibody
      • Kinesin heavy chain neuron-specific 2 antibody
      • Kinesin5C antibody
      • KINN antibody
      • MGC111478 antibody
      • NKHC 2 antibody
      • NKHC antibody
      • NKHC-2 antibody
      • NKHC2 antibody
      see all

    图片

    • Western blot - Anti-KIF5C antibody (ab5630)
      Western blot - Anti-KIF5C antibody (ab5630)

      Shows a Western blot of KIF5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)
      IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab5630?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5630 被引用在 14 文献中.

    • Brachet A  et al. A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression. Cell Rep 36:109499 (2021). PubMed: 34348158
    • Zhang W  et al. Formin Activity and mDia1 Contribute to Maintain Axon Initial Segment Composition and Structure. Mol Neurobiol 58:6153-6169 (2021). PubMed: 34458961
    • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
    • Zhao J  et al. Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. Elife 9:N/A (2020). PubMed: 31961321
    • Leca I  et al. A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse. PLoS Genet 16:e1009104 (2020). PubMed: 33137126
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-KIF5C antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris precast gel)
    Loading amount
    5 µg
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 24 2019

    Western blot abreview for Anti-KIF5C antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Human SH-SY5Y neuroblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Adelheid Hanes

    Verified customer

    提交于 Nov 16 2010

    Immunoprecipitation abreview for Anti-KIF5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (forebrain)
    Immuno-precipitation step
    Protein A
    Specification
    forebrain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Kieran Brickley

    Verified customer

    提交于 May 14 2009

    Western blot abreview for Anti-Kinesin 5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Recombinant protein (HEK 293 cells)
    Loading amount
    50 µg
    Specification
    HEK 293 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 27 2009

    Western blot abreview for Anti-KIF5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    100 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 27 2009

    Question

    Dear Abcam technical team, I want to order proteins from your website, but there are some questions before order. (1) I want to experiment with conventional kinesin heavy chain, KIF5. But there are three different members of kinesin family 5. KIF5A, KIF5B and KIF5C. I know the function of each member, A and C involves in neuronal transport otherwise B involves in inner cell transport. But is there any structural difference between three members? If is, I have to choose specific member to experiment. (2) There are two types in KIF5A and three types in KIF5B. It seems that each has different domain, so I wonder there are functional or structural difference between types. Also, your company has two different types of KIF5C. One is just (938-957) domain and the other is His-tagged protein. His tagged kinesin has higher protein purity. Is there any reason to choose His tagged KIF5C except higher purity? (3) Is antibody that has specificity of my protein (e.g. KIF5A --> Anti-kinesin 5A antibody) attach to specific domain (like C-terminal) or it is just randomly attached? For my experiment, it is good for antibody to attach to C-terminal. In other words, is antibody has C-terminal specificity? (4) Do you sell biotinylated antibody for kinesin? I want antibody that has biotin coating but your website has no option or products for biotin coating. If you cannot understand my questions, please let me know which parts you cannot understand. Then I will send the more specific explanation for my questions. From

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences. Please refer to the following link to see these differences: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111011-085702-0894-35538860-oy To check out further parameters of these proteins, I can recommend to browse either the expasy site (http://web.expasy.org/ or http://web.expasy.org/protparam/) as well as one of the Bioinformatics institutes homepages, for example the EBI, http://www.ebi.ac.uk/. There are different Tools to predict the protein structures. 2.) We do have currently only one KIF5 protein in our catalogue, and this is the ab91695 Kinesin 5C protein (Tagged-His Tag) which is not a full length protein but a recombinant fragment corresponding to amino acids 500-957 of Human Kinesin 5C. Click here (or use the following: https://www.abcam.com/Human-Kinesin-5C-protein-fragment-ab91695.html). All the other products are indeed peptides and do correspond to the short amino acid sequence of the numbers in brackets. These peptides are too short to be able to have a function similar to the whole protein, however they can be used for blocking studies with the corresponding antibody. For example ab41784 is a peptide corresponding the sequence 938 to 957 of human KIF5C. Similarly for the other products: KIF5A: - peptide amino acid 1019-1032 is ab23133 and - peptide amino acid 1007-1027 is ab41781 KIF5B - peptide amino acid 376-396 is ab41782 - peptide amino acid 951-963 is ab23262 - peptide ab100817, a few amino acids derived from the sequence between amino acid 350 to 450 3.) Each antibody is different. I have therefore put together the following list: KIF5A, we do have two antibodies: -ab5628 is a polyclonal antibody directed against the C terminus of KIF5A, recognizing the sequences of amino acids 1007 to 1027 of KIF5A. This antibody has also been tested against the KIF5A protein of different species (human, mouse, rat and cow) as well as in immunocytochemistry, immunoprecipitation and Western blot. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5628.html). -ab5958 which is a polyclonal antibody directed against the C terminal sequence (amino acids 1019 - 1032 of KIF5A). This antibody has however not been well characterized as yet, as you see it is one of our Fasttrack antibodies. Please let me know if you would like to know more about this. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5958.html). KIF5B we have 7 antibodies currently: two of them are against a region in the C terminal: ab15705, corresponding to amino acids 951-963 Click here (or use the following: https://www.abcam.com/Kinesin-5B-antibody-ab15705.html). ab97762, corresponding to amino acids 901 - 963 Click here (or use the following: https://www.abcam.com/KIF5B-antibody-ab97762.html). The other KIF5B antibodies are either N terminal (ab42492, immunogen amino acids 7-56), from the central region (ab5629, immunogen amino acids 376-396 and ab91033 region derived from amino acids 350 - 450) or are mouse monoclonal antibodies for which we do not know the epitope (ab110155 and ab11262) KIF5C we only have one antibody: ab5630 which has been raised against a C terminal sequence as well, the amino acids 938 - 957 of the human KIF5C. Click here (or use the following: https://www.abcam.com/KIF5C-antibody-ab5630.html). 4.) Indeed we do currently not have one of these antibodies in biotinylated form. We do have however the Easylink conjugation kits, which are especially designed for easy conjugation of antibodies. We do have such kits also to perform biotin conjugation. You can find more information in this regards under the following website: www.abcam.com/easylink Again, please let me know if you wish more information about this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Oct 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有