Anti-KIF5B抗体(ab5629)
Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-KIF5B抗体
参阅全部 KIF5B 一抗 -
描述
兔多克隆抗体to KIF5B -
宿主
Rabbit -
特异性
This antibody is specific for KIF5B and does not detect other kinesin isotypes. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/250.
|
| WB |
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782).
|
| 说明 |
|---|
|
ICC/IF
1/250. |
|
WB
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782). |
靶标
-
功能
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. -
序列相似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain. -
结构域
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
细胞定位
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons. - Information by UniProt
-
数据库链接
- Entrez Gene: 3799 Human
- Entrez Gene: 16573 Mouse
- Entrez Gene: 117550 Rat
- Omim: 602809 Human
- SwissProt: P33176 Human
- SwissProt: Q61768 Mouse
- SwissProt: Q2PQA9 Rat
- Unigene: 327736 Human
see all -
别名
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
see all
图片
-
Western blot - Anti-KIF5B antibody (ab5629)All lanes : Anti-KIF5B antibody (ab5629) at 1/2000 dilution
Lane 1 : Mouse brain Whole cell extract
Lane 2 : Mouse lung Whole cell extract
Lane 3 : Rat brain Whole cell extract
Lane 4 : Rat liver Whole cell extract
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L),HRP at 1/4000 dilution
Predicted band size: 110 kDa -
Western blot - Anti-KIF5B antibody (ab5629)All lanes : Anti-KIF5B antibody (ab5629) at 1/4000 dilution
Lane 1 : Mouse Cerebellum whole cell extract
Lane 2 : Rat Brain whole cell extract
Lane 3 : Mouse Brain whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
实验方案
数据表及文件
-
Datasheet download
文献 (15)
ab5629 被引用在 15 文献中.
- Correia SC et al. Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenergetic Profile in the Triple Transgenic Mouse Model of Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33466445
- Pegg CE et al. Herpesviruses assimilate kinesin to produce motorized viral particles. Nature 599:662-666 (2021). PubMed: 34789877
- Lee M et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219:N/A (2020). PubMed: 31910261
- Jiang M et al. Microtubule motor transport in the delivery of melanosomes to the actin-rich apical domain of the retinal pigment epithelium. J Cell Sci 133:N/A (2020). PubMed: 32661088
- Nardi F et al. Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF. Lab Invest 99:1822-1834 (2019). PubMed: 31409893
