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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin

Anti-KIF5B抗体(ab5629)

  • Datasheet
Reviews (2) Submit a question References (15)

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Western blot - Anti-KIF5B antibody (ab5629)
  • Western blot - Anti-KIF5B antibody (ab5629)
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)

Key features and details

  • Rabbit polyclonal to KIF5B
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-KIF5B antibody [EPR10276(B)] (ab167429)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-KIF5B抗体
    参阅全部 KIF5B 一抗
  • 描述

    兔多克隆抗体to KIF5B
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific for KIF5B and does not detect other kinesin isotypes.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human KIF5B aa 376-396.
    Sequence:

    IDEQFDKEKANLEAFTVDKDI


    Database link: P33176
    (Peptide available as ab41782)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human KIF5B peptide (ab41782)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5629于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
1/250.
WB
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782).
说明
ICC/IF
1/250.
WB
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782).

靶标

  • 功能

    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • 序列相似性

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.
  • 结构域

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • 细胞定位

    Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Target information above from: UniProt accession P33176 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3799 Human
    • Entrez Gene: 16573 Mouse
    • Entrez Gene: 117550 Rat
    • Omim: 602809 Human
    • SwissProt: P33176 Human
    • SwissProt: Q61768 Mouse
    • SwissProt: Q2PQA9 Rat
    • Unigene: 327736 Human
    • Unigene: 223744 Mouse
    • Unigene: 219283 Rat
    • Unigene: 4046 Rat
    see all
  • 别名

    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • kinesin 1 (110-120kD) antibody
    • Kinesin 1 heavy chain antibody
    • Kinesin family member 5B antibody
    • Kinesin heavy chain antibody
    • kinesin, heavy chain, ubiquitous antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS 1 antibody
    • KNS antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

图片

  • Western blot - Anti-KIF5B antibody (ab5629)
    Western blot - Anti-KIF5B antibody (ab5629)
    All lanes : Anti-KIF5B antibody (ab5629) at 1/2000 dilution

    Lane 1 : Mouse brain Whole cell extract
    Lane 2 : Mouse lung Whole cell extract
    Lane 3 : Rat brain Whole cell extract
    Lane 4 : Rat liver Whole cell extract

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L),HRP at 1/4000 dilution

    Predicted band size: 110 kDa

  • Western blot - Anti-KIF5B antibody (ab5629)
    Western blot - Anti-KIF5B antibody (ab5629)
    All lanes : Anti-KIF5B antibody (ab5629) at 1/4000 dilution

    Lane 1 : Mouse Cerebellum whole cell extract
    Lane 2 : Rat Brain whole cell extract
    Lane 3 : Mouse Brain whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

    Predicted band size: 110 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)

    ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (15)

发表研究结果有使用 ab5629?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5629 被引用在 15 文献中.

  • Correia SC  et al. Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenergetic Profile in the Triple Transgenic Mouse Model of Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33466445
  • Pegg CE  et al. Herpesviruses assimilate kinesin to produce motorized viral particles. Nature 599:662-666 (2021). PubMed: 34789877
  • Lee M  et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219:N/A (2020). PubMed: 31910261
  • Jiang M  et al. Microtubule motor transport in the delivery of melanosomes to the actin-rich apical domain of the retinal pigment epithelium. J Cell Sci 133:N/A (2020). PubMed: 32661088
  • Nardi F  et al. Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF. Lab Invest 99:1822-1834 (2019). PubMed: 31409893
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Kinesin 5B antibody

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (forebrain)
Total protein in input
20000 µg
Specification
forebrain
Immuno-precipitation step
Protein A
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Kieran Brickley

Verified customer

提交于 May 01 2009

Immunocytochemistry/ Immunofluorescence abreview for Anti-Kinesin 5B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (oligodendrocyte cell line Olineu)
Specification
oligodendrocyte cell line Olineu
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 30 minute(s) · Concentration: 6%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. katarina trajkovic

Verified customer

提交于 Feb 22 2006

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